Identification and characterization of two highly homologous lysozymes from red swamp crayfish, Procambarus clarkii
文献类型： 外文期刊
作者： Yin Cheng-Ming 1 ; Li Ning-Qiu 3 ; Ren Li-Chao 1 ; Wang Zhe 2 ; Chai Lian-Qin 2 ; Lan Jiang-Feng 1 ;
作者机构： 1.Shandong Agr Univ, Coll Anim Sci & Vet Med, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China
2.Henan Univ, State Key Lab Cotton Biol, Sch Life Sci, Kaifeng 475004, Peoples R China
3.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Key Lab Fishery Drug Dev, Key Lab Aquat Anim Immune Technol,Minist Agr, Guangzhou 510380, Peoples R China
关键词： Lysozyme; White spot syndrome virus; Procambarus clarkii; Antimicrobial peptides
期刊名称：FISH AND SHELLFISH IMMUNOLOGY REPORTS
ISSN： 2667-0119
年卷期： 2021 年 2 卷
页码：
收录情况： SCI
摘要： Lysozyme is an important immune effector in innate immunity against pathogen infection. But the study on the active region of lysozyme is limited. In this study, two highly homologous lysozymes were identified from crayfish (designated as PcLysi4 and PcLysi5). The molecular structures of PcLysi4 and PcLysi5 were predicted by SWISS-MODEL with the structure of lysozyme (PDB accession No. 4PJ2.2.B) as model. The results suggested that the structure of PcLysi4 and PcLysi5 were highly similar, but there were more a-helices at positions (127-139) and longer beta-sheet at positions (49-57) in the structure of PcLysi5 than in that of PcLysi4. The antibacterial and antiviral functions of the two lysozymes were investigated. PcLysi4 and PcLysi5 could promote the bacterial clearance ability of crayfish, and increase the survival rate of Vibrio-infected crayfish. Further study showed that PcLysi5 inhibited WSSV replication, and enhanced the survival rate of WSSV-infected crayfish. There was no evidence that PcLysi4 has an influence on WSSV replication. Furthermore, PcLysi5 was detected to interact with envelope protein VP24 of WSSV. Our results would provide a new reference for the study on active region of lysozyme.
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