Ectopic expression of Arabidopsis thaliana Na+(K+)/H+ antiporter gene, AtNHX5, enhances soybean salt tolerance
文献类型: 外文期刊
作者: Wu, X. X. 1 ; Li, J. 1 ; Wu, X. D. 1 ; Liu, Q.; Wang, Z. K. 1 ; Liu, S. S. 1 ; Li, S. N. 1 ; Ma, Y. L. 1 ; Sun, J.; Zhao, 1 ;
作者机构: 1.Northeast Agr Univ, Coll Agron, Key Lab Soybean Biol, Chinese Minist Educ, Harbin, Heilongjiang, Peoples R China
关键词: AtNHX5;Soybean;Na+(K+)/H+ antiporters;Salt tolerance
期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )
ISSN: 1676-5680
年卷期: 2016 年 15 卷 2 期
页码:
收录情况: SCI
摘要: Drought and salt stresses are the two major factors influencing the yield and quality of crops worldwide. Na+(K+)/ H+ antiporters (NHXs) are ubiquitous membrane proteins that play important roles in maintaining the cellular pH and Na+(K+) homeostasis. The model plant Arabidopsis potentially encodes six NHX genes, namely AtNHX1 to 6. In the present study, AtNHX5, a comparatively less well-studied NHX, was cloned and transferred into a soybean variety, Dongnong-50, via Agrobacterium-mediated cotyledonary node transformation to assess its role in improving salt tolerance of the transgenic plants. The transgenic soybean plants were tolerant to the presence of 300 mM NaCl whereas the non-transgenic plants were not. Furthermore, after NaCl treatment, the transgenic plants had a higher content of free proline but lower content of malondialdehyde compared to the non-transgenic plants. Our results revealed that that AtNHX5 possibly functioned by efficiently transporting Na+ and K+ ions from the roots to the leaves. Overall, the results obtained in this study suggest that soybean salt tolerance could be improved through the over expression of Arabidopsis AtNHX5.
- 相关文献
作者其他论文 更多>>
-
EFFECTS OF GENIPOSIDE ON THE REGULATION MECHANISMS OF PHOTOSYNTHETIC PHYSIOLOGY, ROOT OSMOSIS AND HORMONE LEVELS IN MAIZE UNDER SALINE- ALKALI STRESS
作者:Liu, Z. Y.;Gu, W. R.;Li, J.;Wei, S.;Xu, W. H.;Li, C. F.;Wang, M. Q.;Zhang, L. G.;Piao, L.;Zhu, Q. R.
关键词:maize seedlings; photosynthetic enzyme; osmotic substance; antioxidant enzyme; hormone
-
GROWTH OF RYEGRASS (LOLIUM PERENNE L.)
作者:Sun, M. L.;Li, T.;Li, D. M.;Zhao, Y. L.;Gao, F. M.;Sun, L. F.;Sun, G. Y.;Sun, G. Y.
关键词:Glomus mosseae; cadmium stress; plant growth; nutrition accumulation; photosynthetic characteristics
-
Physiological and Transcriptome Analyses of Transgenic FGF21 Immature Rice Seeds
作者:Feng, M. F.;Cai, H.;Wu, X. J.;Yu, D.;Huang, X. Y.;Li, B. W.;Lv, J. H.;Wang, A. X.;Zhang, L. G.;Sun, J.
关键词:Oryza sativa; physiologic tests; RNA-seq; Agrobacterium-mediated transformation; FGF21; transgenic immature rice seeds
-
Effect of extrusion variables on the extrudate characteristics of fish muscle-soy protein blend and optimization of these variables
作者:Zhao, G.;Bi, W.;Zhao, G.;Bi, W.;Liu, H.;Li, J.;Bi, W.;Zhao, G.;Zhang, G.;Yang, C.;Shi, Y.;Zhao, C.;Sun, X.
关键词:Degree of texturization; Extrusion parameter; Fish muscle-soy protein; Twin-screw extruder
-
Role of Chitosan in the Regulation of the Growth, Antioxidant System and Photosynthetic Characteristics of Maize Seedlings under Cadmium Stress
作者:Qu, D. Y.;Gu, W. R.;Li, C. F.;Li, J.;Lia, L. J.;Xie, T. L.;Wei, S.;Zhang, L. G.;Chen, X. C.;Li, J.
关键词:Zea mays; maize; chitosan; cadmium stress; antioxidant enzymes; photosynthetic characteristics
-
Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean
作者:Sun, J.;Li, J.;Liu, M.;Zhang, B. B.;Li, D. M.;Wang, M.;Zhang, C.;Li, W. B.;Wu, X. X.;Sun, J.;Su, A. Y.
关键词:Soybean;Suppression subtractive hybridization (SSH);cDNA library;Soybean regeneration
-
Discovery and transmission of functional QTL in the pedigree of an elite soybean cultivar Suinong14
作者:Yang, R. Q.;Qin, J.;Li, Y. H.;Guan, R. X.;Chang, R. Z.;Qiu, L. J.;Qin, J.;Jiang, C. X.;Li, W. B.
关键词:genotype;phenotype;agronomic trait;pleiotropic effect;intraclass correlation;exotic germplasm;ecologic environment