文献类型： 外文期刊

作者： Zhao, X. H. ¹ ; Li, M. Y. ² ; Xu, S. S. ² ; Liu, G. J. ² ;

作者机构： 1.Heilongjiang Acad Agr Sci, Postdoctoral Programme, Harbin 150086, Heilongjiang, Peoples R China

2.Heilongjiang Acad Agr Sci, Anim Husb Res Inst, Harbin 150086, Heilongjiang, Peoples R China

关键词： Jinghai Yellow chicken;IGFBP-2 gene;PCR-SSCP;Polymorphism;Body weight

期刊名称：BRAZILIAN JOURNAL OF POULTRY SCIENCE （ 影响因子：0.826； 五年影响因子：1.178 ）

ISSN： 1516-635X

年卷期： 2015 年 17 卷 4 期

页码：

收录情况： SCI

摘要： Insulin-like growth factor binding protein-2 (IGFBP-2) regulates a broad spectrum of biological activities involved in growth, development, and differentiation. This study aimed at comparing polymorphisms in intron2 of the IGFBP-2 gene among four chicken breeds and at analyzing the associations between its genotypes and body weight in Jinghai Yellow chicken by using PCR-SSCP technique. For primer P2, three genotypes (AA, AB and BB) were observed in the four chicken breeds. Gene sequencing revealed one insertion/deletion (the inserted/deleted TC after position 552bp) in the intron 2 of IGFBP-2 gene. For primer P5, three genotypes were identified in Jinghai Yellow chickens, and named CC, CD and DD. Gene sequencing revealed two SNPs (C1107G, C1130T) and one inserted/deleted GCCAGGT after 1115bp in the intron 2 of IGFBP-2 gene. The results of the linear model analysis showed that Jinghai Yellow chickens with AA genotype had significantly heavier body weight, at hatch and 12 weeks of age, than those of the AB genotype (p<0.05). The A allele had a positive effect on body weight. We speculate that mutations in intron 2 could be used as genetic markers for body weight in Jinghai Yellow chicken. This study provides valuable information for the protection of genetic resources and for breeding of Jinghai Yellow chicken.