DEVELOPMENT OF THREE-VITRIFICATION-BASED CRYOPRESERVATION PROCEDURES FOR SHOOT TIPS OF CHINA'S POTATO
文献类型： 外文期刊
作者： Wang, Biao 1 ; Wang, Ren-rui 1 ; Li, Jing-wei 1 ; Ma, Yan-li 1 ; Sheng, Wan-min 3 ; Li, Min-fu 2 ; Wang, Qiao-chun 1 ;
作者机构： 1.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Reg, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Inspect & Quarantine, Inst Anim & Plant Quarantine, Beijing 100029, Peoples R China
3.Heilongjiang Acad Agr Sci, Keshan State Potato Germplasm Ctr, Keshan 161606, Heilongjiang, Peoples R China
关键词： Long-term conservation;droplet-vitrification;encapsulation-vitrification;Solanum tuberosum;survival;regrowth
期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）
ISSN： 0143-2044
年卷期： 2013 年 34 卷 4 期
页码：
收录情况： SCI
摘要： China is now the largest potato producing country worldwide. The establishment of efficient conservation techniques for potato germplasm is a prerequisite for breeding of elite cultivars. Potato viral diseases have been a great threat for sustainable potato production in China. Use of virus-free seed tubers is an effective and practical means to control viral diseases. In the present study, three vitrification-based cryopreservation techniques, i.e. droplet-vitrification, encapsulation-vitrification and vitrification were successfully developed for the first time for China's potato. Cultivar `Zihuabai' was used to optimize key parameters involved in the three vitrification-based procedures. With the optimized parameters, shoot regrowth percentages of 71%, 76% and 43% were obtained for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. The three protocols developed were further tested with eight China's major cultivars, with average shoot regrowth of 61%, 38% and 28% for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. Successful development of the three cryopreservation procedures using a single cultivar will facilitate a number of comparative studies such as cryo-injury, regrowth pattern, genetic stability and efficiency of virus elimination. Testing these three cryogenic procedures for potato major cultivars representing a wide range of genetic background, will help the establishment of potato cryobanking in China and the production of virus-free plants.
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