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Fluorescence Spectroscopic Characteristics of Fulvic Acid from the Long-Term Located Fertilization in Black Soil

文献类型： 外文期刊

作者： Li Yan-ping 1 ; Wei Dan 2 ; Zhou Bao-ku 2 ; Zhao Yue 1 ; Zhang Xi-lin 2 ; Wei Zi-min 1 ; Li Shu-ling 1 ;

作者机构： 1.NE Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Inst Soil Fertilizer & Environm Res, Harbin 150086, Peoples R China

关键词： Long-term located fertilization;Black soil;Fulvic acid;Fluorescence spectrum;Integration

期刊名称：SPECTROSCOPY AND SPECTRAL ANALYSIS （ 影响因子：0.589； 五年影响因子：0.504 ）

ISSN： 1000-0593

年卷期： 2011 年 31 卷 10 期

页码：

收录情况： SCI

摘要： In order to investigate the effect of long-term located fertilization on soil fulvic acid (FA), in this study, four soil samples were taken from black soil with long-term located fertilization (about 30 year) in Harbin, Heilongjiang province. The fertilization treatments included control (CK), N, P and K fertilization (NPK), horse manure (OM), combination of organic manure and chemical fertilizations (MNPK). Soil FA was extracted from the samples and purified. The excitation, emission, synchronous, and three-dimensional-excitation emission matrix fluorescence spectroscopy (3DEEM) characteristics of the FA were determined. The excitation, emission and synchronous scan spectra all indicated that the main peaks of FA in the NPK treatment exhibited a significantly blue shift compared with CK, while those of MNPK, OM treatment caused a red shift to some extent. 3DEEM spectra of FA in all treatments exhibited four peaks (peak a, peak b, peak c, and peak d), compared with FA in CK, the wavelengths shift tendency of peak a, peak b, and peak c of FA 3DEEM in NPK, MNPK and OM treatments were similar to that of traditional spectra in FA. In order to provide quantitative information of FA humification degree in different treatments, we investigated the fluorescence index f(450/500) (FI), area integration (A(370 similar to 600nm), A(1 370 similar to 412nm) A(4) (538 similar to 600nm)). Compared with CK, the f(450/500), ratio of A(1)/A in NPK and A(4)/A in MNPK treatment increased by 4. 62%, 6. 12%, 7. 22%, respectively. Howev er, the f(450/500), the ratio of A(1)/A in MNPK and A(4)/A in NPK treatment decreased by 3. 86%, 15. 31%, and 7. 22% respectively. This indicated that NPK application gave a lower degree of FA humification, and combination of organic manure and chemical fertilizations would lead to a greater degree of FA aromatization in black soil with long-term located fertilization than CK.

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