文献类型： 外文期刊

作者： Wang Jing ¹ ; Liu Chun-yan ² ; Zhang Li-wei ¹ ; Wang Jia-lin ¹ ; Hu Guo-hua ² ; Ding Jun-jie ³ ; Chen Qing-shan ¹ ;

作者机构： 1.NE Agr Univ, Coll Agr, Harbin 150030, Peoples R China

2.Land Reclamat Res & Breeding Ctr, Harbin 150090, Peoples R China

3.Heilongjiang Acad Agr Sci, Jiamusi Branch, Jiamusi 154007, Peoples R China

关键词： microRNA (miRNA);microarray;soybean (Glycine max);gene expressional regulation

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2011 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Phytophthora root rot is one of the most prevalent diseases in the world, which can infect the seedlings and plants, with substantial negative impact on soybean yield and quality. MicroRNAs (miRNAs) are a class of post-transcriptional regulators of gene expression during growth and development of organisms. A soybean disease-resistance variety Suinong 10 was inoculated with Phytophthora sojae race No. 1, and the specific miRNA resistant expression profile was acquired by microarray for the first time. Different expressional miRNAs have been found after comparing the results of the treated sample with the-Control sample. Furthermore, the target genes of different expressional miRNAs were predicted. Two miRNAs, cbr-mir-241 and ath-miR854a, regulated the disease-resistance process directly through their targets, some enzymes. Another two miRNAs, gma-miR169a and ath-miR169h, participated in disease-resistance regulation as transcription factors. Similarly, one miRNA, ptc-miR164f, has been reported to regulate the plant development. All of these studies would be served as the foundation for exploring the resistance mechanism.