Antifungal peptide produced by Paenibacillus polymyxa BRF-1 isolated from soybean rhizosphere
文献类型： 外文期刊
作者： Chen, Xueli 1 ; Wang, Guanghua 1 ; Xu, Meina 1 ; Jin, Jian 1 ; Liu, Xiaobing 1 ;
作者机构： 1.Chinese Acad Sci, NE Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Harbin 150081, Peoples R China
2.Heilongjiang Acad Agr Sci, Inst Soil Fertil & Environm Resources, Key Lab Soil Environm & Plant Nutr Heilongjiang P, Harbin 150086, Peoples R China
关键词： Paenibacillus polymyxa;Biolog identification;16S rDNA;antifungal activity;peptide
期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）
ISSN： 1996-0808
年卷期： 2010 年 4 卷 24 期
页码：
收录情况： SCI
摘要： A bacterial strain BRF-1 that produced antifungal peptide was isolated from soybean rhizosphere and identified as Paenibacillus polymyxa by combination approaches of morphological and physiological tests, Biolog identification and 16S rDNA sequencing. Its cell free metabolite can inhabit many plant pathogenic fungi growth and its activity was stable to proteinase K digestion, autoclave and lower pH treatments. The antifungal substance was separated by (NH(4))(2)SO(4) and purified by Sephadex G-50, and acquired short peptide with molecular weight about 35.4 kDa, which has antifungal activity against Rhizoctonia solani.
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