Identification of Δ9 and Δ11 Desaturases Involved in Sex Pheromone Biosynthesis in Mythimna loreyi (Lepidoptera: Noctuidae)
文献类型： 外文期刊
作者： Wang, Liuyang 1 ; Guo, Pei 2 ; Zhang, Xiaofang 2 ; Duan, Yun 4 ; Ning, Jun 3 ; Zhang, Tao 2 ; Yang, Xinling 1 ;
作者机构： 1.China Agr Univ, Coll Sci, Innovat Ctr Pesticide Res, Dept Appl Chem, Beijing 100193, Peoples R China
2.Minist Agr & Rural Affairs, China IPM Innovat Ctr Hebei Prov, Int Sci & Technol Joint Res Ctr IPM Hebei Prov, Plant Protect Inst,HAAFS Key Lab IPM Crops Norther, Baoding 071000, Peoples R China
3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
4.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Peoples R China
关键词： desaturases; unsaturated fatty acids; RNA interference; mating behavior; yeast expression; biosyntheticpathway
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 19 期
页码：
收录情况： SCI
摘要： In moths, sex pheromones are synthesized in pheromone glands (PGs) by a variety of enzymes. Desaturases (DESs) are critical for the introduction of double bonds into pheromones. In Mythimna loreyi, the specific DESs involved in sex pheromone biosynthesis remain unclear. In this study, we identified and characterized 25 putative DESs from the M. loreyi genome. Nineteen of them were expressed in the female PGs, with seven showing significant upregulation in response to pheromone biosynthesis activating neuropeptide (PBAN). RNAi-based knockdown of MlorDES2 significantly reduced the titer of pheromone components by 58.6-85.9%, while knockdown of MlorDES9 specifically reduced the production of (Z)-9-tetradecenyl acetate. Functional verification in yeast revealed that MlorDES2 and MlorDES9 exhibited Delta 11 and Delta 9 desaturase activities, respectively. Taken together, these results collectively demonstrate that MlorDES2 and MlorDES9 are involved in sex pheromone biosynthesis of M. loreyi, suggesting that DESs could be used as potential targets for pest management.
- 相关文献
作者其他论文 更多>>
-
Characterization of the ligand-binding properties of odorant-binding protein 38 from Riptortus pedestris when interacting with soybean volatiles
作者：Guo, Jianglong;Liu, Panjing;Zhang, Xiaofang;An, Jingjie;Li, Yaofa;Zhang, Tao;Gao, Zhanlin
关键词：Riptortus pedestris; OBPs; soybean volatiles; fluorescence competitive binding; molecular docking
-
Three antenna-specific odorant binding proteins in Protaetia brevitarsis (Coleoptera: Scarabaeoidea) involve in recognition of floral volatiles
作者：Liu, Panjing;Wei, Hongyi;Liu, Panjing;Zhang, Xiaofang;Zhang, Tao;Wei, Hongyi;Liu, Panjing;Xiao, Beibei;Wei, Hongyi;Wang, Yubo;Su, Qianfu
关键词：White-spotted flower chafer; OBPs; Fluorescence competitive binding; Key acid residues; Olfactory recognition
-
Manipulation of glycine-serine and flavanone metabolism to maintain plasma membrane stability and improve drought tolerance of millet
作者：Wang, Mengjiao;Guo, Jixun;Zhang, Tao;Shi, Lianxuan;Wang, Bianyin;Liu, Yajie;Zhang, Wenying;Wang, Bianyin;Liu, Yajie;Zhang, Wenying;Zhang, Xiao
关键词：Drought; Metabolomics; Millet; Roots; Transcriptomics
-
A combination of predator and selective pesticide improves control of resistant Aphis gossypii (Hemiptera: Aphididae)
作者：Guo, Pei;Wang, Liuyang;Niu, Yiping;An, Jingjie;Zhang, Tao;Miao, Pu;Li, Chengjun;Li, Shujun
关键词：Cotton aphid; Orius sauteri; afidopyropen; pesticide; natural enemy
-
Antenna-Specific TabsOBP45 and TabsOBP46 Mediate Plant Volatile Recognition in Tuta absoluta (Lepidoptera: Gelechiidae)
作者：Liu, Qingyu;Wang, Liuyang;Ning, Jun;Wang, Liuyang;Liu, Panjing;Zhang, Tao;Li, Lingrui
关键词：the tomato leaf miner; semiochemicals; fluorescence competitive binding; molecular docking; host volatile recognition
-
Identification of Female Sex Pheromone of a Plant Bug, Polymerus pekinensis Reuter (Hemiptera: Miridae)
作者：Wang, Liuyang;Zhang, Xiaofang;Zhang, Tao;Wang, Yubo;Fang, Meijuan;Mei, Xiangdong
关键词：Miridae; chemical communication; octyl acetate; decyl acetate; trapping
-
Exploring repellency of odors from non-host plants native to Xinjiang, China to Aphis gossypii
作者：Bian, Zhipeng;Yuan, Haibin;Zhang, Ying;Liu, Bing;Lu, Yanhui;Zhang, Tao
关键词：
Aphis gossypii ; gas chromatography-mass spectrometry (GC-MS); electroantennogram (EAG); volatile compounds; repellent effects
