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Three antenna-specific odorant binding proteins in Protaetia brevitarsis (Coleoptera: Scarabaeoidea) involve in recognition of floral volatiles

文献类型： 外文期刊

作者： Liu, Panjing 1 ; Zhang, Xiaofang 2 ; Wang, Yubo 4 ; Xiao, Beibei 3 ; Su, Qianfu 5 ; Zhang, Tao 2 ; Wei, Hongyi 1 ;

作者机构： 1.Jiangxi Agr Univ, Minist Educ, Key Lab Crop Physiol Ecol & Genet Breeding, Nanchang, Peoples R China

2.Minist Agr & Rural Affairs, Plant Protect Inst, Key Lab IPM Crops Northern Reg North China, HAAFS, Beijing, Peoples R China

3.Jiangxi Agr Univ, Sch Agron Sci, Nanchang, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Dry Land Farming Inst, Hengshui, Peoples R China

5.Jilin Acad Agr Sci, Northeast Agr Res Ctr China, Inst Plant Protect, Gong Zhuling 136100, Peoples R China

关键词： White-spotted flower chafer; OBPs; Fluorescence competitive binding; Key acid residues; Olfactory recognition

期刊名称：JOURNAL OF INSECT PHYSIOLOGY （ 影响因子：2.3； 五年影响因子：2.5 ）

ISSN： 0022-1910

年卷期： 2025 年 164 卷

页码：

收录情况： SCI

摘要： Odorant binding proteins (OBPs) play a vital role in the insect olfactory recognition system, as they bind and transport specific semiochemicals to chemosensory receptors for further processing. The agricultural and horticultural pest, white-spotted flower chafer (WSFC), Protaetia brevitarsis (Coleoptera, Scarabaeoidea), has significantly harmed numerous crops and fruits in China. It is hypothesized that WSFCs rely on specific OBPs and chemosensory receptors to identify palatable food sources and optimal oviposition sites. Twenty-three putative OBPs (PbreOBPs) were identified from the WSFC genome in our research. Based on phylogenetic analysis, PbreOBPs exhibited high degree of similarity to those of Holotrichia parallela and Anomala corpulenta. Tissue expression profiles showed that ten PbreOBPs exhibited antenna-biased expression. We recombined and purified three antenna-specific PbreOBPs: PbreOBP8, PbreOBP9, and PbreOBP18. Fluorescence competitive binding assays revealed that they had higher binding affinities to beta-ionone, phenethyl salicylate, phenylacetaldehyde, and benzyl benzoate - compounds commonly found in floral volatiles. Additionally, PbreOBP8 showed the ability to bind microbial volatiles, such as 3-octanol, 3-methyl-1-butanol, and 1-octen-3-ol, suggesting a role in locating food or oviposition sites. Forthermore, we also predicted the key acid residues which involved in the binding of three PbreOBPs towards floral and microbial volatiles. The present investigation demonstrated that the antenna-specific PbreOBP8, PbreOBP9 and PbreOBP18 were likely involved in mediating the recognition of floral volatiles. The findings introduce possible avenues for further research into PbreOBPs and provide potential targets for creating olfactory-based control methods for WSFC populations.

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