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Transcriptomic Analysis of Hippocampus abdominalis Larvae Under High Temperature Stress

文献类型： 外文期刊

作者： Xiao, Wenjie 1 ; Guo, Baoying 2 ; Tan, Jie 1 ; Liu, Changlin 1 ; Jiang, Da 1 ; Yu, Hao 1 ; Geng, Zhen 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Zhejiang Ocean Univ, Marine Sci & Technol Coll, Zhoushan 316022, Peoples R China

关键词： Hippocampus abdominalis; larvae; temperature stress; transcriptome; RT-qPCR

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN：

年卷期： 2024 年 15 卷 10 期

页码：

收录情况： SCI

摘要： Objectives: Acute temperature stress was explored in Hippocampus abdominalis through a comprehensive RNA-seq analysis. Methods: RNA-seq was conducted on 20-day-old H. abdominalis after 24 h of temperature stress. Four experimental conditions were established: a control group (18 degrees C) and three temperature treatment groups (21, 24, and 27 degrees C). Results: Seahorse larvae were found to be unaffected by 21 degrees C and 24 degrees C and were able to survive for short periods of time during 24 h of incubation, whereas mortality approached 50% at 27 degrees C. The sequencing process produced 75.63 Gb of high-quality clean data, with Q20 and Q30 base percentages surpassing 98% and 96%, respectively. A total of 141, 333, and 1598 differentially expressed genes were identified in the 21, 24, and 27 degrees C groups vs. a control comparison group, respectively. Notably, the number of up-regulated genes was consistently higher than that of down-regulated genes across all comparisons. Gene Ontology functional annotation revealed that differentially expressed genes were predominantly associated with metabolic processes, redox reactions, and biosynthetic functions. In-depth KEGG pathway enrichment analysis demonstrated that down-regulated genes were significantly enriched in pathways related to steroid biosynthesis, terpenoid backbone biosynthesis, spliceosome function, and DNA replication. Up-regulated genes were enriched in pathways associated with the FoxO signaling pathway and mitophagy (animal). The results indicated that temperature stress induced extensive changes in gene expression in H. abdominalis, involving crucial biological processes such as growth, biosynthesis, and energy metabolism. Conclusions: This study provided key molecular mechanisms in the response of H. abdominalis to temperature stress, offering a strong basis for future research aimed at understanding and mitigating the effects of environmental stressors on marine species.

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