A kelch domain cell end protein, PoTea1, mediates cell polarization during appressorium morphogenesis in Pyricularia oryzae
文献类型： 外文期刊
作者： Qu, Yingmin 1 ; Cao, Huijuan 2 ; Huang, Pengyun 3 ; Wang, Jing 3 ; Liu, Xiaohong 1 ; Lu, Jianping 3 ; Lin, Fu-Cheng 1 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310058, Zhejiang, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China
3.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Pyricularia oryzae; Tea1Polarity; Appressorium formation; Pmk1 MAPK pathway; Virulence
期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.07； 五年影响因子：7.103 ）
ISSN： 0944-5013
年卷期： 2022 年 259 卷
页码：
收录情况： SCI
摘要： The rice blast fungus Pyricularia oryzae differentiates into an infection structure, called an appressorium, for plant penetration. The process of appressorium formation requires the transformation of polarized growth to isotropic growth, while penetration requires the opposite growth transformation from isotropic to polarized. Polarized growth requires coordinated organization of cytoskeletal elements, such as microtubule and actin. We identified PoTea1, a homolog of Tea1 from Schizosaccharomyces pombe, and characterized its roles in P. oryzae. After PoTEA1 deletion, Delta Potea1 displayed slowed hyphal growth, decreased sporulation, increased hyphal branches, abnormal two-celled spores, and reduced plant penetration and virulence. During appressorium formation, Delta Potea1 developed a long germ tube with a small appressorium, leading to delayed appressorium differentiation and reduced glycogen and lipid droplet degradation. Delta Potea1 is defective in cAMP-PKA and Pmk1 MAPK pathways. PoTea1 localized at hyphal tips and appressoria as bright dots and was highly dynamic during appressorium formation. PoTea1 formed a complex with itself by self-assembly that was highly dependent on its kelch motif. The coiled-coil motif C2 of PoTea1 is involved in self polymerization and appressorium formation. Benomyl and latrunculin A, two cytoskeleton inhibitors, disturbed the stable localization of PoTea1 at vegetative hyphal tips. We speculate that PoTea1 functions in appressorium formation and virulence by mediating cell polarity in P. oryzae.
- 相关文献
作者其他论文 更多>>
-
Development of an LC-MS Method for the Quantitative Determination of Six Organic Acids Produced by Bacterial Fermentation In Vitro and In Vivo
作者：Wang, Jing;Tang, Xuxiao;Qu, Chao;Zhang, Yingzhong;Zhang, Chaoqun;Chu, Qiulu;Wang, Hao
关键词：organic acids; extraction; LC-MS; quantification; in vitro; in vivo
-
Rapid and Visual Detection of Pyricularia oryzae Using Coupled Recombinase Polymerase Amplification-Lateral Flow Dipstick Assay
作者：Du, Yan;Qi, Zhongqiang;Yu, Junjie;Zhang, Rongsheng;Yu, Mina;Cao, Huijuan;Song, Tianqiao;Pan, Xiayan;Wang, Shuchen;Liu, Yongfeng;Yang, Jun;Xiong, Qin;Liu, Yongfeng
关键词：disease diagnosis; lateral flow dipstick;
Pyricularia oryzae ; recombinase polymerase amplification -
Synergistic Cooling Effect of Metal-Doped Red-Orange Pigments: One-Step Synthesis for Enhanced Near-Infrared Reflectance and Selective Infrared Emission
作者：Lin, Lin;Zhang, Bocheng;Zha, Keyu;Tao, Shuang;Fang, Liang;Lu, Chunhua;Xu, Zhongzi;Lin, Lin;Zhang, Bocheng;Zha, Keyu;Tao, Shuang;Fang, Liang;Lu, Chunhua;Xu, Zhongzi;Lin, Lin;Zhang, Bocheng;Zha, Keyu;Tao, Shuang;Fang, Liang;Lu, Chunhua;Xu, Zhongzi;Yin, Jialiang;Wang, Jing;Zhao, Mizhen
关键词：inorganic pigments; NIR reflectance; Li2MnO3; cooling coatings
-
Woronin body protein UvHex1 is essential for pathogenesis, and contributes to conidiation, cell stress response, lipid metabolism, and mycotoxins production in rice false smut fungus Ustilaginoidea virens
作者：Mao, Yijun;Deng, Qingchao;Yu, Junjie;Mao, Yijun;Pan, Xiayan;Yu, Mina;Cao, Huijuan;Song, Tianqiao;Qi, Zhongqiang;Du, Yan;Zhang, Rongsheng;Liu, Yongfeng;Yu, Junjie
关键词：
Ustilaginoidea virens ; Woronin body; UvHex1; Pathogenicity -
Integration of ATAC-Seq and RNA-Seq Reveals the Role of FaTIP1 in Red Light-Induced Fruit Ripening in Strawberry
作者：Chen, Xiaodong;Cai, Weijian;Xia, Jin;Wang, Jing;Yuan, Huazhao;Wang, Qinglian;Pang, Fuhua;Zhao, Mizhen;Qiao, Yushan
关键词：aquaporin; LED light; strawberry; chromatin accessibility
-
Characterization and comparative analysis of volatile organic compounds in four aromatic wild strawberry species using HS-SPME-GC-MS
作者：Xu, Linlin;Liu, Wan;Pan, Zhiliang;Pang, Fuhua;Zhang, Yongqi;Liang, Jiahui;Wang, Qinglian;Wang, Jing;Zhao, Mizhen;Qiao, Yushan;Yuan, Huazhao;Xu, Linlin;Wang, Qinglian;Wang, Jing;Qiao, Yushan;Yuan, Huazhao;Liu, Wan;Pan, Zhiliang
关键词：Aroma; Gas chromatography-mass spectrometry; (GC-MS); Strawberry germplasm; Volatile organic compounds (VOCs)
-
Optimized straw incorporation depth can improve the nitrogen uptake and yield of rapeseed by promoting fine root development
作者：Wang, Chunyun;Wang, Zongkai;Lou, Hongxiang;Wang, Xianling;Shao, Dongli;Tan, Xiaoqiang;Liu, Mengzhen;Wang, Bo;Kuai, Jie;Wang, Jing;Xu, Zhenghua;Zhou, Guangsheng;Zhao, Jie;Wang, Chunyun;Gao, Jianqin;Zhang, Jiefu
关键词：Straw incorporation; Rapeseed; Nitrogen; Root; Yield
