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Identification of candidate genes for easily-shelled traits in Tartary buckwheat based on BSA-Seq and RNA-Seq methods

文献类型： 外文期刊

作者： Duan, Ying 1 ; Yin, Guifang 2 ; He, Runli 1 ; Yang, Xiaolin 1 ; Cai, Suyun 1 ; Wang, Yanqing 2 ; Lu, Wenjie 2 ; Sun, Daowang 2 ; Wang, Lihua 2 ; Wang, Yongqin 3 ; Sun, Honghe 3 ;

作者机构： 1.Shanxi Univ Chinese Med, Coll Tradit Chinese Med & Food Engn, Taiyuan 030619, Shanxi, Peoples R China

2.Yunnan Acad Agr Sci, Yunnan Prov Key Lab Agr Biotechnol, Key Lab Southwestern Crop Gene Resources & Germpl, Minist Agr & Rural Affairs Biotechnol & Genet Ger, Kunming 650201, Yunnan, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr Vegetable Res Ctr, Beijing 100081, Peoples R China

关键词： Tartary buckwheat; Easy dehulling; BSA-Seq; RNA-Seq; KASP

期刊名称：EUPHYTICA （ 影响因子：2.185； 五年影响因子：2.387 ）

ISSN： 0014-2336

年卷期： 2022 年 218 卷 7 期

页码：

收录情况： SCI

摘要： The hard-shelled character of ordinary cultivated Tartary buckwheat has become a factor influencing its taste and nutritional efficacy. However, the local variety, Rice-Tartary, can dehull easily. The genetic mechanism regulating easily-shelled Fagopyrum tataricum is unknown. In this study, the F-2 generation segregating population was constructed by crossing Yunqiao No.1 (hard-shelled) and Rice-Tartary (easily-shelled) as parents, and the bulked segregant analysis sequencing (BSA-seq) strategy was used to initial mapping. The gene locus controlling the easily-shelled trait of Tartary buckwheat was preliminarily located in the 4.07 Mb region of the first chromosome. To further narrow the range, the Kompetitive allele-specific PCR primers based on the single nucleotide polymorphisms in the initial location range were designed and tested in 335 individual plants in the hybrid F-2 population. The candidate gene Ftes1 was located between Ft6,705,225 and Ft7,041,921, according to the genetic linkage map constructed based on typing data. Combined with the location of candidate genes, the RNA-Seq data, and qRT-PCR results, the results indicate that 3 genes, FtPinG0001427400.01, FtPinG0001428600.01, and FtPinG0002492200.01 may regulate the easily-shelled Tartary buckwheat. The results of this study provide vital candidate genes for the cloning and functional analysis of genes related to easily-shelled traits, as well as prominent molecular markers for breeding of new varieties of Tartary buckwheat.

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