Cold stress disrupts gill homeostasis in juvenile yellowfin tuna (Thunnus albacares) by altering oxidative, metabolic, and immune responses
文献类型： 外文期刊
作者： Huang, Junhua 1 ; Fu, Zhengyi 1 ; Bai, Jing 8 ; Ma, Zhenhua 1 ;
作者机构： 1.Sanya Trop Fisheries Res Inst, Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572018, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China
3.Sanya Trop Fisheries Res Inst, Hainan Engn Res Ctr Deep Sea Aquaculture & Proc, Sanya 572018, Peoples R China
4.Sanya Trop Fisheries Res Inst, Int Joint Res Ctr Conservat & Applicat Fishery Res, Sanya 572018, Peoples R China
5.Chinese Acad Fishery Sci, Hainan Fisheries Innovat Res Inst, Sanya 572024, Peoples R China
6.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
7.Flinders Univ S Australia, Coll Sci & Engn, Adelaide 5001, Australia
8.Hainan Shuancheng Marine Technol Dev Co Ltd, Haikou 570100, Peoples R China
关键词： Low-temperature stress; Gill tissue; Enzyme activity analysis; Gene expression; Histological analysis
期刊名称：MARINE ENVIRONMENTAL RESEARCH （ 影响因子：3.2； 五年影响因子：3.3 ）
ISSN： 0141-1136
年卷期： 2025 年 210 卷
页码：
收录情况： SCI
摘要： Acute cold stress can disrupt physiological homeostasis in marine fish and may induce pronounced metabolic and immune responses in pelagic species such as yellowfin tuna (Thunnus albacares), which possess regional endothermic capabilities. As a key tissue interfacing with the environment, the gill plays essential roles in gas exchange, ion regulation, immune defense, and energy metabolism, making it highly susceptible to thermal fluctuations. This study investigated the physiological responses of gill tissue in juvenile yellowfin tuna under acute cold stress, using two treatment groups-LT (24 degrees C) and ULT (18 degrees C)-with a control group (CG, 30 degrees C). Sampling was conducted at 0, 12, 24, and 36 h to assess antioxidant and metabolic enzyme activities, histopathological alterations, and the expression of immune- and metabolism-related genes. Results showed time-dependent changes in antioxidant enzymes (SOD, CAT, POD, GSH-Px), with significantly elevated MDA and LPO levels at 12 h and 24 h (p < 0.05), especially under 18 degrees C, indicating intensified oxidative stress. Significant alterations in AST, LDH, ACP, and AKP suggested metabolic reprogramming and membrane function changes. Variations in Na+/K+-ATPase and Ca2+/Mg2+-ATPase activity reflected ion regulation under thermal stress. Gene expression of hspa8b, heat shock cognate 71 kDa protein, irf3, lpl, and pck2 demonstrated temperature- and time-dependent adaptive responses. Histological analysis revealed progressive edema, necrosis, and lamellar disruption with decreasing temperature. Collectively, these findings highlight a multilayered stress response in yellowfin tuna gill tissue under cold exposure and provide new insights into thermal adaptation and cold-tolerant aquaculture strategies for pelagic species.
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