文献类型： 外文期刊

作者： Wang, Dehao ¹ ; Wang, Liying ¹ ; Bi, Dexi ⁴ ; Song, Jipeng ¹ ; Wang, Guohao ¹ ; Gao, Ye ¹ ; Tang, Kathy F. J. ¹ ; Meng, Fanzeng ¹ ; Xie, Jingmei ¹ ; Zhang, Fan ¹ ; Huang, Jie ¹ ; Li, Jianliang ² ; Dong, Xuan ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

2.Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An, Shandong, Peoples R China

3.Shanghai Ocean Univ, Shanghai, Peoples R China

4.Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Pathol, Shanghai, Peoples R China

5.Tianjin Agr Univ, Tianjin, Peoples R China

6.Network Aquaculture Ctr Asia Pacific, Bangkok, Thailand

7.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China

8.Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao, Peoples R China

9.QingdaoKey Lab Mariculture Epidemiol & Biosecur, Qingdao, Peoples R China

关键词： acute hepatopancreatic necrosis disease (AHPND); type IV secretion system (T4SS); conjugative transfer; pVA1-type plasmid

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：9.043； 五年影响因子：8.113 ）

ISSN： 2165-0497

年卷期：

页码：

收录情况： SCI

摘要： The pathogenic pVA1-type plasmids that carry pirAB toxin genes are the genetic basis for Vibrio to cause acute hepatopancreatic necrosis disease (AHPND), a lethal shrimp disease posing an urgent threat to shrimp aquaculture. Emerging evidence also demonstrate the rapid spread of pVA1-type plasmids across Vibrio species. The pathogenic pVA1-type plasmids that carry pirAB toxin genes are the genetic basis for Vibrio to cause acute hepatopancreatic necrosis disease (AHPND), a lethal shrimp disease posing an urgent threat to shrimp aquaculture. Emerging evidence also demonstrate the rapid spread of pVA1-type plasmids across Vibrio species. The pVA1-type plasmids have been predicted to encode a self-encoded type IV secretion system (T4SS). Here, phylogenetic analysis indicated that the T4SS is a novel member of Trb-type. We further confirmed that the T4SS was able to mediate the conjugation of pVA1-type plasmids. A trbE gene encoding an ATPase and a traG gene annotated as a type IV coupling protein (T4CP) were characterized as key components of the T4SS. Deleting either of these 2 genes abolished the conjugative transfer of a pVA1-type plasmid from AHPND-causing Vibrio parahaemolyticus to Vibrio campbellii, which was restored by complementation of the corresponding gene. Moreover, we found that bacterial density, temperature, and nutrient levels are factors that can regulate conjugation efficiency. In conclusion, we proved that the conjugation of pVA1-type plasmids across Vibrio spp. is mediated by a novel T4SS and regulated by environmental factors. IMPORTANCE AHPND is a global shrimp bacteriosis and was listed as a notifiable disease by the World Organization for Animal Health (WOAH) in 2016, causing losses of more than USD 7 billion each year. Several Vibrio species such as V. parahaemolyticus, V. harveyi, V. campbellii, and V. owensii harboring the virulence plasmid (designated as the pVA1-type plasmid) can cause AHPND. The increasing number of Vibrio species makes prevention and control more difficult, threatening the sustainable development of the aquaculture industry. In this study, we found that the horizontal transfer of pVA1-type plasmid is mediated by a novel type IV secretion system (T4SS). Our study explained the formation mechanism of pathogen diversity in AHPND. Moreover, bacterial density, temperature, and nutrient levels can regulate horizontal efficiency. We explore new ideas for controlling the spread of virulence plasmid and form the basis of management strategies leading to the prevention and control of AHPND.