文献类型： 外文期刊
作者： Sun, Xiujun 1 ; Li, Li 2 ; Wu, Biao 1 ; Ge, Jianlong 1 ; Zheng, Yanxin 3 ; Yu, Tao 3 ; Zhou, Liqing 1 ; Zhang, Tianshi 1 ; Y 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pilot Natl Lab Marine Sci & Technol Qingdao, Minist Agr & Rural Affairs,Key Lab Sustainable De, Yellow Sea Fisheries Res Inst,Lab Marine Fisherie, Qingdao 266071, Peoples R China
2.Marine Biol Inst Shandong Prov, Qingdao 266104, Peoples R China
3.Chinese Acad Fishery Sci, Changdao Enhancement & Expt Stn, Yantai 265800, Peoples R China
关键词： Scallop; Adductor muscle; Cell-type diversity; Mesenchymal stem cell; Single-cell trajectories
期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）
ISSN： 0888-7543
年卷期： 2021 年 113 卷 6 期
页码：
收录情况： SCI
摘要： Studies on cell atlas in marine invertebrates provide a better understanding of cell types, stem cell maintenance, and lineages of cell differentiation. To investigate the molecular features of various cell types in molluscan muscles, we performed single-cell RNA sequencing (scRNA-seq) to map cell types in scallop adductor muscles. We uncovered the cell type-specific features of 20 cell clusters defined by the expression of multiple specific molecular markers. These cell clusters are mainly classified into four broad classes, including mesenchymal stem cells, muscle cells, neurons, and haemolymph cells. In particular, we identified a diverse repertoire of neurons in the striated adductor muscle, but not in the smooth muscle. We further reconstructed the cell differentiation events using all the cell clusters by single-cell pseudotemporal trajectories. By integrating dual BrdU-PCNA immunodetection, neuron-specific staining and electron microscopy observation, we showed the spatial distribution of mesenchymal stem cells and neurons in striated adductor muscle of scallops. The present findings will not only be useful to address the cell type-specific gene expression profiles in scallop muscles, but also provide valuable resources for cross-species comparison of marine organisms.
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