您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Pseudorabies gD protein protects mice and piglets against lethal doses of pseudorabies virus

文献类型： 外文期刊

作者： Zhao, Mengpo 1 ; Chen, Jing 1 ; Luo, Shengjun 1 ; Yan, Renhe 4 ; Zhang, Pian 1 ; Ren, Zhaowen 1 ; Chen, Xiaofan 1 ; Wang, Gang 1 ; Xiang, Hua 1 ; Cai, Rujian 1 ; Huang, Yuan 1 ; Li, Na 1 ; Li, Hongwei 5 ; Yuan, Zi-Guo 3 ; Wang, Xiaohu 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Key Lab Livestock Dis Prevent Guangdong Prov, Sci Observat, Guangzhou, Peoples R China

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Key Lab Prevent & Control Avian Influenza & Other, Minist Agr & Rural Affairs, Guangzhou, Peoples R China

3.South China Agr Univ, Coll Vet Med, Guangzhou, Peoples R China

4.Guangzhou Bioneeds Biotechnol Co LTD, Dept Res & Dev, Guangzhou, Peoples R China

5.Southern Med Univ, Sch Lab & Biotechnol, Guangzhou, Peoples R China

关键词： pseudorabies virus; 293T cells; recombinant gD protein; subunit vaccine; immune protection

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： IntroductionPseudorabies (PR) is a highly contagious viral disease caused by the pseudorabies virus (PRV), which can cause disease in a wide range of domestic and wild animals. Studies have shown that new mutant strains have emerged in pig farms in many regions and that commercial inactivated and live attenuated vaccines are becoming less effective at protecting pigs.MethodsPorcine pseudorabies glycoprotein D (gD) gene (GenBank: QEY95774.1) with hexa-His tag to the C terminus for further purification processes was cloned into the lentiviral expression plasmid pLV-CMV-eGFP by restriction enzyme, the resulting plasmid was designated as pLV-CMV-gD. HEK-293T cells with robust and stable expression of recombinant gD protein was established by infection with recombinant lentivirus vector pLV-CMV-gD. We expressed porcine pseudorabies virus gD protein using HEK-293T cells.ResultsWe describe in this study that individual gD proteins produced by a mammalian cell expression system are well immunogenic and stimulate high levels of PRV-specific and neutralizing antibodies in mice and piglets. All mice and piglets survived lethal doses of PRV, significantly reducing the amount of PRV virus in piglets' lymph nodes, lungs, spleen, and other tissues. It also significantly reduced the time cycle and amount of viral excretion from piglets to the environment through the nasal and anal cavities.DiscussionThe results suggest that PRV gD protein is expected to be a potential candidate for the preparation of genetically engineered PR vaccines for the prevention of PRV infection and the control of PR epidemics.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台