文献类型： 外文期刊

作者： Wang, Haoran ¹ ; Chao, Shufen ¹ ; Yan, Qing ¹ ; Zhang, Shu ³ ; Chen, Guoqing ¹ ; Mao, Chonghui ¹ ; Hu, Yang ⁴ ; Yu, Fengquan ⁵ ; Wang, Shuo ⁶ ; Lv, Liang ³ ; Yang, Baojun ¹ ; He, Jiachun ¹ ; Zhang, Songbai ⁷ ; Zhang, Liangsheng ⁸ ; Simmonds, Peter ¹⁰ ; Feng, Guozhong ¹ ;

作者机构： 1.Chinese Acad Agr Sci, China Natl Rice Res Inst, State Key Lab Rice Biol & Breeding, Hangzhou 311400, Peoples R China

2.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

3.Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertilizer, Wuhan 430064, Peoples R China

4.Guizhou Acad Agr Sci, Inst Plant Protect, Guiyang 550000, Peoples R China

5.Liaoning Acad Agr Sci, Inst Plant Protect, Shenyang 110161, Peoples R China

6.Sanya Agr Technol Extens & Serv Ctr, Sanya 572000, Peoples R China

7.Yangtze Univ, Coll Agr, Jingzhou 434000, Peoples R China

8.Fujian Agr & Forestry Univ, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Peoples R China

9.Zhejiang Univ, Coll Agr & Biotechnol, Genom & Genet Engn Lab Ornamental Plants, Hangzhou 310012, Peoples R China

10.Univ Oxford, Nuffield Dept Med, Peter Medawar Bldg Pathogen Res, Oxford OX1 3SY, England

关键词： invertebrate species; metatranscriptome; viral diversity; viral transmission; viral genomes

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：9.1； 五年影响因子：7.1 ）

ISSN： 1674-7305

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Invertebrate species are a natural reservoir of viral genetic diversity, and invertebrate pests are widely distributed in crop fields. However, information on viruses infecting invertebrate pests of crops is limited. In this report, we describe the deep metatranscriptomic sequencing of 88 invertebrate samples covering all major invertebrate pests in rice fields. We identified 296 new RNA viruses and 13 known RNA viruses. These viruses clustered within 31 families, with many highly divergent viruses constituting potentially new families and genera. Of the identified viruses, 13 RNA viruses clustered within the Fiersviridae family of bacteriophages, and 48 RNA viruses clustered within families and genera of mycoviruses. We detected known rice viruses in novel invertebrate hosts at high abundances. Furthermore, some novel RNA viruses have genome structures closely matching to known plant viruses and clustered within genera of several plant virus species. Forty-five potential insect pathogenic RNA viruses were detected in invertebrate species. Our analysis revealed that host taxonomy plays a major role and geographical location plays an important role in structuring viral diversity. Cross-species transmission of RNA viruses was detected between invertebrate hosts. Newly identified viral genomes showed extensive variation for invertebrate viral families or genera. Together, the large-scale metatranscriptomic analysis greatly expands our understanding of RNA viruses in rice invertebrate species, the results provide valuable information for developing efficient strategies to manage insect pests and virus-mediated crop diseases.