Elevated CO2 alters antibiotic resistome in soil amended with sulfamethazine via chemical-organic fertilization
文献类型： 外文期刊
作者： Xu, Fen 1 ; Xiang, Qian 3 ; Xu, Mei-Ling 1 ; Carter, Laura J. 5 ; Du, Wen-Chao 6 ; Zhu, Chun-Wu 7 ; Ai, Fu-Xun 1 ; Yin, Ying 1 ; Ji, Rong 1 ; Guo, Hong-Yan 1 ;
作者机构： 1.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Peoples R China
2.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Hubei Key Lab Nutr Qual & Safety Agroprod, Wuhan 430064, Peoples R China
3.Chinese Acad Sci, Key Lab Urban Environm & Hlth, Inst Urban Environm, Xiamen 361021, Peoples R China
4.CAS Haixi Ind Technol Innovat Ctr Beilun, Zhejiang Key Lab Urban Environm Proc & Pollut Cont, Ningbo 315830, Peoples R China
5.Univ Leeds, Fac Environm, Sch Geog, Leeds LS2 9JT, England
6.Nanjing Normal Univ, Sch Environm, Nanjing 210023, Peoples R China
7.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China
关键词： Antibiotics resistance genes; Free-air CO2 enrichment; Sulfamethazine; Soil bacterial community; Chemical-organic fertilizer
期刊名称：ENVIRONMENTAL RESEARCH （ 影响因子：7.7； 五年影响因子：7.7 ）
ISSN： 0013-9351
年卷期： 2025 年 264 卷
页码：
收录情况： SCI
摘要： Rising antimicrobial resistance (AMR) is an enormous challenge for global healthcare systems. The effects of elevated CO2 (eCO2) on AMR are poorly characterized. Using a free-air CO2 enrichment system and high- throughput qPCR arrays, we investigated the response of soil antibiotic resistome and bacterial communities to eCO2 (ambient + 200 ppm) in soils amended with sulfamethazine (SMZ) at 0.1 and 1 mg kg-1 via chemical- organic fertilizer (COL, COH). Results showed that under ambient condition, COH significantly enhanced the diversity of high-risk antibiotic resistance genes (ARGs), relative abundance of low risk ARGs, unassessed ARGs and total ARGs compared to COL. Nevertheless, eCO2 mitigated the effects of COH, with no significant difference found between COL and COH on the above high risk, low risk, unassessed and total ARGs. Meanwhile, eCO2 decreased the relative abundance of spcN, ermA, olec, oprD, sulA-olP, tetB, tetT and vanXD in COL, and alleviated the enrichment of pikR2, ampC, lunC, oprD and pncA caused by the application of SMZ at 1 mg kg-1. Correlation and network analysis illustrated that changes of certain bacteria biomarkers and horizontal gene transfer of integrase gene were associated with the altered response of ARGs abundance to eCO2. This study adds knowledge of the potential risk of antibiotic resistance in agricultural exposure scenarios under increasing CO2 concentration.
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