文献类型： 外文期刊

作者： Batool, Saneya ¹ ; Bukhari, Syed Mohsin Raza ² ; Ling, Peng ³ ; Zahid, Anam ⁴ ; Fozia, Ehab A. A. ⁵ ; Salama, Ehab A. A. ⁵ ; Alrefaei, Abdulwahed Fahad ⁶ ; Almutairi, Mikhlid H. ⁶ ; Ahmed, Mohamed A. A. ⁷ ;

作者机构： 1.Hainan Univ, Coll Life & Hlth Sci, Dept Ornamental Hort & Landscape, Haikou, Hainan, Peoples R China

2.Univ Agr Faisalabad, Fac Food Sci & Technol, Dept NIFSAT, Faisalabad, Punjab, Pakistan

3.Hainan Univ, Coll Forestry, Dept Ornamental Hort & Landscape, Haikou 570228, Hainan, Peoples R China

4.Beijing Forestry Univ, Coll Landscape Architecture, Natl Flower Engn Res Ctr, Beijing Key Lab Ornamental Plants Germplasm Innova, Beijing, Peoples R China

5.Alexandria Univ, Fac Agr Saba Basha, Agr Bot Dept Genet, Alexandria, Egypt

6.King Saud Univ, Coll Sci, Dept Zool, Riyadh, Saudi Arabia

7.Alexandria Univ, Fac Agr Saba Basha, Plant Prod Dept Hort Med & Aromat Plants, Alexandria 21531, Egypt

8.Chinese Acad Trop Agr Sci, Coconut Res Inst, Natl Key Lab Trop Crop Breeding, Wenchang, Hainan, Peoples R China

关键词： Nodal explant; organogenesis; rooting; virus-free culture; yellow passion fruit; Agriculture & Environmental Sciences; Botany; Plant & Animal Ecology

期刊名称：COGENT FOOD & AGRICULTURE （ 影响因子：1.7； 五年影响因子：2.7 ）

ISSN： 2331-1932

年卷期： 2024 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Yellow passion fruit (Passiflora edulis f. flavicarpa) is valuable edible specie with economic significance in China. Passion fruit faces yield losses due to viral diseases. This study used tissue culture of nodal shoot segments to propagate virus-free plants. Surface-sterilized nodal shoot segments were used and shoots were initiated on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-Benzyladenine (6-BA) (0.0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1). Multiple shoots were initiated (5.2 +/- 1.09 shoots/explant) with a 6-BA concentration of 2.0 mg L-1. Sub-culturing of these multiple shoots on MS medium augmented with 2.0 mg L-1 6-BA and 0.2 mg L-1 IAA was successful in vitro with maximum numbers of shoots (6.6 +/- 1.17) within four weeks of incubation. These regenerated shoots were assayed for presence of passion fruit woodiness virus (PWV) using enzyme-linked immunosorbent assay (ELISA); only virus-free shoots were used for further propagation. Root formation was very good in each indole-3-butyric acid (IBA) and (IAA) at a concentration of 0.6 mg L-1 with a maximum number of roots (7.2 +/- 1.30). Rooted plantlets were gradually acclimatized in a greenhouse and successfully established in soil. Thus, virus-free plants can be successfully regenerated directly from nodal shoot segments.