您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The phenylcoumaran benzylic ether reductase gene PtPCBER improves the salt tolerance of transgenic poplar through lignan-mediated reactive oxygen species scavenging

文献类型： 外文期刊

作者： Wei, Mingxing 1 ; Yu, Chunyan 2 ; Ge, Bingkun 1 ; Liu, Yue 3 ; Zhang, Haiyang 4 ; Duan, Chunli 1 ; Zhang, Juan 2 ; Mao, Tingting 2 ; Huang, Huiqing 2 ; Xie, Yinshuai 1 ; Gao, Hongsheng 2 ; Zhang, Hongxia 2 ; Wang, Aike 7 ; Yi, Yanjun 1 ;

作者机构： 1.Qingdao Agr Univ, Coll Life Sci, 700 Changcheng Rd, Qingdao 266109, Shandong, Peoples R China

2.Ludong Univ, Engn Res Inst Agr & Forestry, 186 Hongqizhong Rd, Yantai 264025, Shandong, Peoples R China

3.Qingdao Agr Univ, Coll Hort, 700 Changcheng Rd, Qingdao 266109, Shandong, Peoples R China

4.Shandong Inst Sericulture, 21 Zhichubei Rd, Yantai 264001, Shandong, Peoples R China

5.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Hainan Key Lab Biosafety Monitoring & Mol Breeding, 4 Xueyuan Rd, Haikou 571101, Hainan, Peoples R China

6.Ludong Univ, Univ Shandong, Key Lab Mol Module Based Breeding High Yield & Abi, 186 Hongqizhong Rd, Yantai 264025, Shandong, Peoples R China

7.Yucheng Inst Agr Sci, Shangqiu 476000, Henan, Peoples R China

8.186 Hongqizhong Rd, Yantai 264025, Shandong, Peoples R China

9.700 Changcheng Rd, Qingdao 266109, Shandong, Peoples R China

关键词： Salt stress; Lignan; PtPCBER; Poplar; ROS; Transgenic plant

期刊名称：ENVIRONMENTAL AND EXPERIMENTAL BOTANY （ 影响因子：6.028； 五年影响因子：6.246 ）

ISSN： 0098-8472

年卷期： 2022 年 201 卷

页码：

收录情况： SCI

摘要： Phenylcoumaran benzylic ether reductase (PCBER) in the phenylpropane metabolic pathway plays a crucial role in controlling plant growth and development. However, its biological function in response to abiotic stress in perennial trees is still largely obscure. In this work, a phenylcoumaran benzylic ether reductase gene, PtPCBER, isolated from poplar was overexpressed in poplar. The growth and salt resistance of transgenic plants were investigated, and the possible regulatory mechanism of PtPCBER in response to abiotic stresses was verified. PtPCBER was constitutively expressed in various tissues and organs with a predominant expression in xylems. Overexpression of PtPCBER augmented the salt and oxidative stress tolerance of transgenic poplar plants. The increased salt tolerance was associated with a relatively lower chlorphyll loss and Na+ accumulation, and a higher antioxidant enzyme activity and stress gene expression, in the leaves of transgenic plants. Further metabolomic analyses revealed that overexpression of PtPCBER cut down the accumulation of coniferyl alcohols and lignans which function in the reactive oxygen species (ROS) scavenging pathway to alleviate the damage caused by oxidative stress in the leaves of transgenic plants. Taken together, our results suggest that PtPCBER has an important function in plant response to salt stress, and it could be used as an ideal candidate gene for the genetic engineering of woody plants with enhance resistance to multiple abiotic stresses through improving the scavenging capacity of ROS.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台