Function Identification of a Lactonase Gene lac1563 Involved in Producing Urolithin A of Limosilactobacillus fermentum FUA033
文献类型： 外文期刊
作者： Wang, Ze 1 ; Liu, Xiaoli 3 ; Liu, Shu 1 ; Zhou, Wenmei 1 ; Chen, Tao 4 ; Gao, Song 1 ; Wu, Hao 1 ; Zhang, Wenjun 1 ; Tang, Rui 1 ; Fang, Yaowei 1 ;
作者机构： 1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China
2.Jiangsu Ocean Univ, Sch Food Sci & Engn, Lianyungang 222005, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China
4.Suqian Prod Qual Supervis & Testing Inst, Suqian 223814, Peoples R China
关键词： Limosilactobacillus fermentum FUA033; genome mining; lactonase; ellagic acid; urolithin A
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 11 期
页码：
收录情况： SCI
摘要： This study aimed to identify the lactonase gene in Limosilactobacillus fermentum FUA033 that facilitates the conversion of ellagic acid into urolithin A. Lactonase gene candidates were identified genome analysis. A series of overexpression, knockout, and complementation strains of these candidate genes were subsequently generated. The urolithin A yield of the lac1563 knockout strain was significantly reduced compared to the wild-type strain, whereas the overexpression strain exhibited a markedly higher yield. The complementation strain showed no significant difference in urolithin A yield relative to the wild-type strain. In contrast, alterations in the lac329 gene did not significantly impact urolithin A production compared to the wild-type strain. Additionally, the candidate lactonase genes were cloned and heterologously expressed in Escherichia coli. The recombinant lactonase derived from the lac1563 gene, unlike that from the lac329 gene, demonstrated activity toward ellagic acid. Optimal activity of the lac1563 lactonase occurred at 37 degrees C and pH 7.0. Enzyme activity was notably enhanced by Mn2+ and Co2+, while inhibited by Zn2+ and Cu2+. These findings contribute valuable insights into the metabolic pathway of ellagic acid conversion to urolithin A and establish the lac1563 gene as a promising candidate for biotechnological applications aimed at urolithin A production.
- 相关文献
作者其他论文 更多>>
-
Inactivation of Penicillium ochrochloron spores by pulsed light: Mechanism and preservation efficacy in strawberries
作者：Cui, Xiaozhen;Bassey, Anthony Pius;Wang, Fan;Xie, Shudong;Fan, Linlin;Zhu, Yongsheng;Liu, Xiaoli;Cui, Xiaozhen;Xie, Shudong;Li, Dongnan;Bin, Li
关键词：Non-thermal technology; Pulsed light; Strawberry; Fruits; Quality
-
Moderate Nitrogen Application Synergistically Improved Yield and Quality of Nanjing Series japonica Rice Varieties with Good Taste
作者：Wei, Xiaodong;Zhao, Qingyong;Zhao, Chunfang;Zhang, Yong;Chen, Tao;Zhu, Zhen;Lu, Kai;He, Lei;Zhou, Lihui;Huang, Shengdong;Li, Yusheng;Wang, Cailin;Zhang, Yadong;Wang, Cailin;Zhang, Yadong
关键词：nitrogen level; japonica rice; high-quality cultivation; grain yield; good eating quality
-
Maple Syrup Adulteration: Fluorescence Fingerprints as a Source of Information for Enhanced Detection
作者：Singh, Maleeka;Zhang, Maia;Espinal-Ruiz, Mauricio;Corradini, Maria G.;Rathnayake, Sujani;Hanner, Robert;Xue, Jun;Shi, John;Liu, Xiaoli;Corradini, Maria G.;Espinal-Ruiz, Mauricio
关键词：
-
Fabrication and characterization of gelatin/carboxymethyl chitosan composite film incorporated with carvacrol and its preservation efficacy in Chinese mitten crab ( Eriocheir sinensis)
作者：Bassey, Anthony Pius;Cui, Xiaozhen;Wang, Fan;Nasiru, Mustapha Muhammad;Fan, Linlin;Liu, Xiaoli;Ibeogu, Isaiah Henry;Bako, Hadiza Kabir
关键词：Chinese mitten crab; Active packaging; Carvacrol; Bacterial diversity; Preservation; Films
-
Inhibitory effects of phenolic compounds from blueberry leaf on α-amylase and α-glucosidase: kinetics, mode of action, and molecular interactions
作者：Wu, Han;Liu, Xiaoli;Xie, Shudong;Zhou, Jianzhong;Pan, Yue;Cui, Xiaozhen;Wu, Han;Liu, Xiaoli;Zhou, Jianzhong;Wu, Han;Liu, Xiaoli;Corradini, Maria G.;Corradini, Maria G.
关键词：blueberry leaf; digestive enzymes; kinetics; inhibition mechanism; multispectral technique
-
Enhanced viability of Lactiplantibacillus plantarun by encapsulation with whey protein isolate fibrils and polysaccharides through layer-by-layer coating
作者：Li, Siyuan;Su, Wei;Zhang, Yu;Gan, Wanling;Liu, Xiaoli;Fan, Linlin
关键词：Lactiplantibacillus plantarun; Layer-by-layer coating; Whey protein isolate fibrils; Cell adhesion capacity; Hyaluronic acid
-
Screening of hypoglycemic and probiotic properties of Pediococcus acidilactici FM-Pa-JXM94 and exploring the potential hypoglycemic mechanism
作者：Wang, Ying;Zhang, Hui;Wang, Xingna;Zhang, Yu;Fan, Linlin;Ma, Yanhong;Liu, Xiaoli;Wang, Ying;Zhang, Hui;Liu, Xiaoli;Wang, Ying
关键词：Pediococcus acidilactici; alpha-Amylase and alpha-glucosidase inhibition; activities; Lactic acid bacteria; Probiotic properties; Hypoglycemic function
