您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Adaptive responses and metabolic strategies of Novosphingobium sp. ES2-1-17β-estradiol analyzed through integration of genomic and proteomic approaches

文献类型： 外文期刊

作者： Li, Shunyao 1 ; Yang, Wei 1 ; Mo, Jingjing 1 ; Wang, Yubing 1 ; Lu, Chao 4 ; Gao, Yanzheng 2 ; Li, Yucheng 1 ; Sun, Kai 3 ;

作者机构： 1.Anhui Univ, Anhui Prov Key Lab Wetland Ecosyst Protect & Resto, Jiulong Rd 111, Hefei 230601, Peoples R China

2.Nanjing Agr Univ, Inst Organ Contaminant Control & Soil Remediat, Coll Resources & Environm Sci, Nanjing 210095, Peoples R China

3.Anhui Agr Univ, Coll Resources & Environm, Anhui Prov Key Lab Farmland Ecol Conservat & Pollu, Hefei 230036, Anhui, Peoples R China

4.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Natl Agr Expt Stn Agr Environm, Nanjing 210014, Peoples R China

关键词： Microbial degradation; Steroid estrogens; Novosphingobium; Molecular mechanisms; Genomics and proteomics

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2024 年 461 卷

页码：

收录情况： SCI

摘要： Environmental 17 beta-estradiol (E2) can cause potential harm to ecological balance and human health. Novosphingobium sp. ES2-1 is an E2-degrading bacterium previously obtained, which converts E2 to estrone (E1) and then to 4-hydroxyestrone (4-OH-E1) followed by oxidation to form metabolites with long-chain structure during upstream degradation. Herein, we found that intracellular enzymes were the major contributors to E2 biodegradation by strain ES2-1. A total of 243 proteins were dys-expressed under E2 condition, 123 were up-regulated and 120 were down-regulated thereinto. The up-regulated members of ABC transport systems, aromatics degradation, and fatty acid degradation indicated a reinforced transfer and utilization of E2. Cytochrome P450 monooxygenase (EstP1), 2-keto-4-pentenoate hydratase, pyruvate dehydrogenase, acetyl-CoA acetyltransferase, TonB-dependent receptor were involved in E2 catabolism. During downstream degradation, the metabolites with long-chain structure were decomposed adopting beta-oxidation pattern and ultimately entered the TCA cycle; 2 keto-4-pentenoic acid might be an emblematic product of such process. Furthermore, E2 converting to E1 was catalyzed by 17 beta-dehydrogenase probably encoded by IM701_16645 or IM701_16910; 4-OH-E1 meta-cleavage was catalyzed by a dioxygenase encoded by IM701_20340 or IM701_21000 or IM701_09625. Our study provided an in-depth insight into the adaptive responses and metabolic strategies of Novosphingobium to E2.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台