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CircRNA-02191 regulating unsaturated fatty acid synthesis by adsorbing miR-145 to enhance CD36 expression in bovine mammary gland

文献类型： 外文期刊

作者： Chen, Zhi 1 ; Wang, Yuhao 2 ; Wang, Kun 2 ; Zhang, Zhenbin 2 ; Han, Mengli 1 ; Li, Guoqing 1 ; Zhang, Bin 1 ; Yang, Yang 1 ; Loor, Juan J. 3 ; Yang, Zhangping 2 ; Zhong, Fagang 1 ; Dai, Rong 1 ; Wang, Mengzhi 2 ;

作者机构： 1.Xinjiang Acad Agr Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China

2.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Peoples R China

3.Univ Illinois, Dept Anim Sci, Mammalian Nutr Physiol Genom, Urbana, IL 61801 USA

4.Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA

关键词： CircRNA-02191; MiR-145; CD36; Bovine mammary gland; Unsaturated fatty acid

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 244 卷

页码：

收录情况： SCI

摘要： CD36 functions as a receptor for long-chain fatty acids, promoting the absorption and transport of long-chain unsaturated fatty acids. However, the regulatory influence of upstream circRNAs or miRNAs on its expression in cow mammary gland remains unclear. Herein, we performed high-throughput sequencing to screen for differentially expressed miRNAs and mRNAs in bovine mammary tissue during the late-lactation and the dry period to screen and conducted bioinformatics analysis to identify 420 miRNA/mRNA pairs, including miR-145/ CD36. Experimental results indicate that miR-145 can directly target CD36 and inhibit its expression. Addi-tionally, the circRNA-02191 sequence is predicted to contain a miR-145 binding site. As shown by dual luciferase reporter system detection, circRNA-02191 bound to miR-145 and its overexpression significantly reduced the expression of miR-145. Furthermore, the overexpression of miR-145 inhibited triglyceride accumulation, while circRNA-02191 enhanced the expression of the miR-145 target gene CD36. The above results indicate that circRNA-02191 can regulate triglyceride and fatty acid components by binding miR-145 and subsequently alleviating the inhibitory effect of miR-145 on the expression of CD36. Taken together, these findings present a novel approach to improve milk quality by analyzing the regulatory effect and mechanism regulating the circ02191/miR-145/CD36 pathway on fatty acid synthesis in the mammary gland of dairy cows.

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