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Effect of Perilla frutescens polyphenols against tight junction alterations and intestinal inflammation: A Caco-2/macrophage co-culture model-based study integrated with network pharmacology

文献类型： 外文期刊

作者： Hu, Anqi 1 ; Ye, Yuhui 2 ; Han, Binsong 2 ; Zhu, Xiaoai 2 ; Xue, Feng 3 ; Yang, Lifei 1 ; Zhang, Cunzheng 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual, Safety State Key Lab Cultivat Base Minist Sci & Te, Nanjing 210014, Peoples R China

3.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China

关键词： Co-culture; Intestinal inflammation; Network pharmacology; Perilla frutescens; Tight junction

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2024 年 62 卷

页码：

收录情况： SCI

摘要： The intestinal barrier plays important roles in the uptake of nutrients and prevention of harmful pathogens. However, intestinal inflammation causes barrier dysfunction, which may contribute to inflammatory bowel disease (IBD). Polyphenols from Perilla frutescens (L.) Britt. Exhibit various bioactivities, though their protective effect on the intestinal barrier remains unclear. To address this, the study investigated the effect of Perilla frutescens (L.) Britt. polyphenols (PFP) against tight junction alterations and intestinal inflammation using an intestinal-like Caco-2/macrophage co-culture model. LC-MS/MS analysis identified 15 polyphenols in the PFP extract. Furthermore, PFP were transported across the apical Caco-2 monolayer, with apigenin, genistin, syringic acid and cinnamic acid showing relatively high transport rates (>70%). The lipopolysaccharide (LPS)-induced tight junction alterations, evaluated by trans-epithelial electrical resistance (TEER) analysis, qPCR, and immunofluorescence assay, were significantly restored by medium and high doses of PFP (P < 0.05). In addition, network pharmacology analysis demonstrated that the core targets of PFP for intestinal inflammation were mostly located in the MAPK and NF-kappa B signaling pathways. The expression levels of the key proteins in these pathways were further validated by qPCR and western blot analyses. Overall, PFP exhibits great potential as a novel functional food component for treating intestinal inflammation.

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