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Interaction of MaERF11 with the E3 ubiquitin ligase MaRFA1 is involved in the regulation of banana starch degradation during postharvest ripening

文献类型： 外文期刊

作者： Jiang, Mengge 1 ; Yang, Yingying 1 ; Wei, Wei 1 ; Wu, Chaojie 1 ; Shan, Wei 1 ; Kuang, Jianfei 1 ; Chen, Jianye 1 ; Wei, Shouxing 2 ; Lu, Wangjin 1 ;

作者机构： 1.South China Agr Univ, Coll Hort, Engn Res Ctr Southern Hort Prod Preservat, Minist Educ,Guangdong Prov Key Lab Postharvest Sci, Guangzhou 510642, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Hainan, Peoples R China

关键词： Banana; MaERF11; E3 ubiquitin ligase MaRFA1; Fruit ripening; Starch degradation

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：6.1 ）

ISSN： 2095-9885

年卷期： 2025 年 11 卷 2 期

页码：

收录情况： SCI

摘要： Banana fruit ripening is a highly regulatory process involving various layers consisting of transcriptional regulation, epigenetic factor, and post-translational modification. Previously, we reported that MaERF11 cooperated with MaHDA1 to precisely regulate the transcription of ripening-associated genes via histone deacetylation. However, whether MaERF11 is subjected to post-translational modification during banana ripening is largely unknown. In this study, we found that MaERF11 targeted a subset of starch degradation-related genes using the DNA affinity purification sequence (DAP-Seq) approach. Electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter assay (DLR) demonstrated that MaERF11 could specifically bind and repress the expression of the starch degradation-related genes MaAMY3, MaBAM2 and MaGWD1. Further analyses of yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and Luciferase complementation imaging (LCI) assays indicated that MaERF11 interacted with the ubiquitin E3 ligase MaRFA1, and this interaction weakened the MaERF11-mediated transcriptional repression capacity. Collectively, our results suggest an additional regulatory layer in which MaERF11 regulates banana fruit ripening and expands the regulatory network in fruit ripening at the post-translational modification level.

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