您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

PbrCalS5, a callose synthase protein, is involved in pollen tube growth in Pyrus bretschneideri

文献类型： 外文期刊

作者： Cao, Peng 1 ; Tang, Chao 1 ; Wu, Xiao 1 ; Qian, Ming 1 ; Lv, Shouzheng 1 ; Gao, Hongru 1 ; Qiao, Xin 1 ; Chen, Guodong 1 ; Wang, Peng 1 ; Zhang, Shaoling 1 ; Wu, Juyou 1 ;

作者机构： 1.Nanjing Agr Univ, Ctr Pear Engn Technol Res, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

2.Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Peoples R China

3.Huaiyin Inst Technol, Coll Life Sci & Food Engn, Huaian 223003, Peoples R China

4.Nanjing Agr Univ, Sanya Inst, Sanya 572024, Peoples R China

关键词： CalS; Evolution; Pear; Callose; Pollen tube

期刊名称：PLANTA （ 影响因子：4.54； 五年影响因子：4.689 ）

ISSN： 0032-0935

年卷期： 2022 年 256 卷 2 期

页码：

收录情况： SCI

摘要： Main conclusion Identification of CalS genes in seven Rosaceae species and functional characterization of PbrCalS5 in pear pollen tube growth by regulating callose deposition. Callose exists widely in angiosperms and has significant functions in a range of developmental processes. Callose is synthesized by callose synthase (CalS). However, the members of the callose synthase gene family and their evolutionary profiles, along with their biological functions, in species of the Rosaceae remain unknown. In this study, a total of 69 members of the CalS gene family in seven Rosaceae species (Fragaria vesca, Malus x domestica, Prunus avium, Pyrus bretschneideri, Prunus mume, Prunus persica and Rubus occidentalis) were identified and divided into six clades. Different types of gene duplication events contributed to the expansions of the CalS gene family in the seven species, with purifying selection playing a key role in the evolution of the CalS genes. Tissue-specific expression patterns analysis revealed that PbrCalS5 was highly expressed in the pear pollen tube and was selected for further functional analysis. Subcellular localization indicated that PbrCalS5 was localized in the plasma membrane and cell wall. Antisense oligodeoxynucleotide (AS-ODN) assays resulted in the inhibition of PbrCalS5 expression, leading to the decreased callose deposition in the pollen tube wall and subsequent inhibition of pear pollen tube growth. These results provide the theoretical basis for exploring the functional roles of CalS genes in pear pollen tube growth.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台