A rapid and highly sensitive CRISPR assay utilizing Cas12a orthologs for the detection of Novel Duck Reovirus
文献类型： 外文期刊
作者： Wang, Yuan 1 ; Fu, Lanting 1 ; Li, Sheng 1 ; Tao, Dagang 1 ; Gong, Ping 4 ; Yang, Yu 4 ; Ruan, Jinxue 1 ; Xie, Shengsong 1 ; Wang, Cui 3 ; He, Daqian 3 ;
作者机构： 1.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Key Lab Swine Genet & Breeding, Minist Agr & Rural Affairs, Wuhan 430070, Peoples R China
3.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China
4.Wuhan Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430208, Peoples R China
关键词： NDRV; Cas12a orthologs; Gs12-16; Gs12-18; CRISPR-based diagnostics
期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）
ISSN： 0039-9140
年卷期： 2026 年 297 卷
页码：
收录情况： SCI
摘要： The Novel Duck Reovirus (NDRV) seriously threatens the global poultry industry due to the lack of effective therapies. Preventive measures for NDRV heavily depend on early disease detection, highlighting the need for rapid and sensitive diagnostic methods. This study used Cas12a orthologs Gs12-18 to develop a visual CRISPRbased detection assay targeting the NDRV S3 gene. Comparative analysis of candidate Cas12a proteins, Gs12-16 and Gs12-18, showed that Gs12-18 has significantly better trans-cleavage activity, making it especially suitable for highly sensitive nucleic acid detection. We integrated Gs12-18 with loop-mediated isothermal amplification (LAMP) technology to create a LAMP-CRISPR/Gs12-18 detection platform. This method enables visual detection of the NDRV S3 gene with high specificity and sensitivity, with a detection limit of 38 copies per reaction. It does not require complex equipment and is suitable for point-of-care testing. This research provides a reliable diagnostic tool for the early prevention and control of NDRV.
- 相关文献
作者其他论文 更多>>
-
Exploring the Role of Edible Dock Powder (Rumex K-1) in Enhancing Growth Performance, Organ Health, and Cecal Microbiota in Sanhua Goslings
作者：Wang, Xianze;Wang, Junliang;Liu, Yi;Li, Guangquan;Gong, Shaoming;Wang, Huiying;He, Daqian
关键词：Chinese gosling; edible grass; growth metrics; serum biochemical markers; cecal microbial diversity
-
Impact of calsporin® ( Bacillus subtilis C-3102) supplementation on growth performance and intestinal function in geese
作者：Li, Guangquan;Wang, Huiying;Wang, Xianze;He, Daqian;Yang, Lei;Xu, Guangpei
关键词：Goose; Growth performance; Intestinal; Microbiota
-
Genetic and metabolic mechanisms underlying webbed feet pigmentation in geese: Insights from histological, transcriptomic, and metabolomic analyses
作者：Liu, Yi;Weng, Kaiqi;Li, Guangquan;Wang, Huiying;He, Daqian;Tan, Yu
关键词：Goose; Transcriptome; Metabolome; Webbed feet color; Mechanisms of pigmentation
-
Unveiling Key Genes and Crucial Pathways in Goose Muscle Satellite Cell Biology Through Integrated Transcriptomic and Metabolomic Analyses
作者：Liu, Yi;Wang, Cui;Yang, Yunzhou;Wang, Huiying;He, Daqian;Li, Mingxia;Chen, Shufang
关键词：goose; skeletal muscle satellite cells; transcriptomic; metabolomic; gene expression; differentiation; PPAR signaling pathway
-
Transcriptomic and Lipidomic Characteristics of Subcutaneous Fat Deposition in Small-Sized Meat Ducks
作者：Zheng, Hao;Zhou, Ao;Wang, Cui;Chen, Xing
关键词：meat duck; subcutaneous fat deposition; lipidomics; transcriptomics; gene expression; adipocytokine signaling pathway
-
Impact of fasting and refeeding on immune markers, hepatic gene expression, and gut microbiota in geese: insights into metabolic regulation and gut-liver interactions
作者：Liu, Yi;Li, Guangquan;Wang, Xianze;He, Daqian;Jia, Huiyan;Dai, Jiuli;Chen, Shufang
关键词：fasting and refeeding; hepatic gene expression; gut microbiota; gut-liver interactions; immune markers; geese
-
Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying Ducks
作者：Xiao, Changfeng;Yang, Changsuo;He, Daqian;Zhu, Lihui;Xu, Yunying
关键词：fermented feed; laying duck; egg quality; intestinal health
