Crucial role of dead end gene for primordial germ cell survival in rice field eel (Monopterus albus)
文献类型： 外文期刊
作者： Hu, Qiaomu 2 ; Xiao, Qing 1 ; Tian, Haifeng 2 ; Li, Dapeng 1 ; Li, Zhong 2 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Hubei Prov Engn Lab Pond Aquaculture, Wuhan 430070, Peoples R China
2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
关键词： Monopterus albus; Dead end; Primordial germ cell; Morpholino; Cas9-gRNA
期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）
ISSN： 0093-691X
年卷期： 2021 年 176 卷
页码：
收录情况： SCI
摘要： The dead end gene has been identified as a essential factor for Primordial germ cells (PGCs) migration and survival in many species, but its role in Monopterus albus is unclear. In order to clarify the function of dead end gene in M.albus PGCs migration and survival, we first characterized the expression profile of M.albus dead end (Madnd) in developing embryos and various tissues. qRT-PCR revealed that Madnd transcripts were exclusively detected in gonad, including ovary, testis and ovotestis.Embryos injected with a Madnd morpholino (Madnd-MO) exhibited down-regulation of the vasa gene. Furthermore, the GFP signal show the PGCs migration in control group were injected with GFP-nanos3 3'-UTR mRNA for visualization, as described in a previous study, yet it was disappeared after embryos injected with Madnd-MO.Finally, we characterized the genomics sequence of the Madnd gene and designed five gRNAs for genome editing. Three gRNAs were selected for microinjection according to the results of in vitro tests. gRNAd1 was used for microinjection with the Cas9 protein and was confirmed to be effective. Our analysis in this study suggested that Madnd play a key role in PGCs migration and survival in M. albus. These data provide the basis for the production of fast-growing and reproductively M.albus sterile. (C) 2021 Published by Elsevier Inc.
- 相关文献
作者其他论文 更多>>
-
Antiviral activity of arctigenin against Chinese rice-field eel rhabdovirus in Monopterus albus
作者：Liu, Yisha;Xue, Mingyang;Xu, Chen;Zhou, Yong;Jiang, Nan;Meng, Yan;Li, Yiqun;Huang, Zhenyu;Liu, Wenzhi;Li, Zhong;Fan, Yuding;Liu, Yisha;Zhong, Qiwang
关键词：Arctigenin; Rhabdovirus; Monopterus albus; Antiviral
-
Molecular Characterization and Expression of unc-13d in the Sex Reversal of Monopterus albus
作者：Lian, Zitong;Meng, Fang;Xia, Xueping;Fang, Junchao;Tian, Haifeng;Hu, Qiaomu;Hu, Qiaomu
关键词：
Monopterus albus ;unc-13d ; expression; in situ hybridization; DNA methylation -
Myomaker and Myomixer are required for craniofacial myoblast fusion in zebrafish
作者：Zhang, Zhanxiong;Yong, Pengzheng;Hu, Qiaomu;Du, Shaojun;Hu, Qiaomu
关键词：Carey-Fineman-Ziter syndrome; craniofacial muscle; myoblast fusion; Myomaker; Myomixer
-
Genetic diversity of Gymnocypris firmispinatus: Insights from microsatellites and mitochondrial DNA among wild, broodstock source, and broodstock populations
作者：He, Huke;Qi, Pengzhi;He, Huke;Tian, Haifeng;Peng, Liang;Zhong, Liqiao;Ma, Baoshan;Wang, Xiaodong;Qi, Pengzhi
关键词：Gymnocypris firmispinatus; Molecular markers; Genetic diversity; Population structure; Stock enhancement release activities
-
Understanding Genetic Regulation of Sex Differentiation in Hermaphroditic Fish
作者：Fang, Junchao;Li, Guanglve;Hu, Qiaomu;Luo, Wenyin;Hu, Qiaomu
关键词：hermaphroditic fish; sex-determining genes; mechanisms of sex differentiation; sexual transition processes; histological alterations
-
Differential Expression Characteristics of Two Isoforms nr5a2f and nr5a2m in Gonadal Differentiation of Chinese Giant Salamanders, Andrias davidianus
作者：Hu, Dan;Li, Guanglve;Xu, Jiaqing;Luo, Wenyin;Hu, Qiaomu;Zou, Guohua
关键词：
Andrias davidianus ; Nr5a2; alternative splicing; differential expression -
Diversification of Toll-like receptor 1 in swamp eel ( Monopterus albus )
作者：Rao, Han;Huo, Caifei;Li, Yan;Tian, Haifeng;Li, Zhong;Wang, Xueting;Zhu, Lilan
关键词：Monopterus albus; Toll -like receptor 1 (TLR1); Monopterus javanensis; Genetic diversity
