Short-chain fatty acids (SCFAs) as potential resuscitation factors that promote the isolation and culture of uncultured bacteria in marine sediments
文献类型： 外文期刊
作者： Sun, Chun-Shui 1 ; Zhou, Liu-Yan 4 ; Liang, Qi-Yun 1 ; Wang, Xiao-Man 5 ; Lei, Yi-Xuan 1 ; Xu, Zhen-Xing 6 ; Wang, Feng-Qing 7 ; Chen, Guan-Jun 1 ; Du, Zong-Jun 1 ; Mu, Da-Shuai 1 ;
作者机构： 1.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China
2.Shandong Univ, Inst Microbial Technol, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China
3.Shandong Univ, Weihai Res Inst Ind Technol, Weihai 264209, Peoples R China
4.Xinjiang Acad Agr Sci, Inst Microbiol Applicat, Urumqi 830000, Peoples R China
5.Tancheng Cty Inspection & Testing Ctr, Tancheng 276100, Peoples R China
6.Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan
7.Max Planck Inst Marine Microbiol, Celsiusstr 1, D-28359 Bremen, Germany
关键词： Marine bacteria; SCFAs; VBNC; Resuscitation culture
期刊名称：MARINE LIFE SCIENCE & TECHNOLOGY （ 影响因子：5.7； 五年影响因子：5.8 ）
ISSN： 2096-6490
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Many marine bacteria are difficult to culture because they are dormant, rare or found in low-abundances. Enrichment culturing has been widely tested as an important strategy to isolate rare or dormant microbes. However, many more mechanisms remain uncertain. Here, based on 16S rRNA gene high-throughput sequencing and metabolomics technology, it was found that the short-chain fatty acids (SCFAs) in metabolites were significantly correlated with uncultured bacterial groups during enrichment cultures. A pure culture analysis showed that the addition of SCFAs to media also resulted in high efficiency for the isolation of uncultured strains from marine sediments. As a result, 238 strains belonging to 10 phyla, 26 families and 82 species were successfully isolated. Some uncultured rare taxa within Chlorobi and Kiritimatiellaeota were successfully cultured. Amongst the newly isolated uncultured microbes, most genomes, e.g. bacteria, possess SCFA oxidative degradation genes, and these features might aid these microbes in better adapting to the culture media. A further resuscitation analysis of a viable but non-culturable (VBNC) Marinilabiliales strain verified that the addition of SCFAs could break the dormancy of Marinilabiliales in 5 days, and the growth curve test showed that the SCFAs could shorten the lag phase and increase the growth rate. Overall, this study provides new insights into SCFAs, which were first studied as resuscitation factors in uncultured marine bacteria. Thus, this study can help improve the utilisation and excavation of marine microbial resources, especially for the most-wanted or key players.
- 相关文献
