文献类型： 外文期刊

作者： Xu, Shisan ¹ ; Zhang, Huan ³ ; Li, Cun-Zhao ⁴ ; Lai, Ping-Shan ⁴ ; Wang, Guijiang ⁵ ; Chan, Yu Suen ⁶ ; Cheng, Shuk Ha ¹ ;

作者机构： 1.Guangxi Normal Univ, Sch Life Sci, Guilin 541004, Peoples R China

2.Guangxi Normal Univ, Guangxi Univ Key Lab Stem Cell & Biopharmaceut Te, Guilin 541004, Peoples R China

3.Vitargent Int Biotechnol Ltd, Shatin, Unit 516,5-F Biotech Ctr 2,11 Sci Pk West Ave, Hong Kong, Peoples R China

4.Natl Chung Hsing Univ, Dept Chem, Taichung 402, Taiwan

5.Heilongjiang Acad Agr Sci, Xuefu Rd 368, Harbin, Heilongjiang, Peoples R China

6.City Univ Hong Kong, Coll Vet Med & Life Sci, Dept Biomed Sci, Hong Kong, Peoples R China

关键词： Cannabidiol; Zebrafish; Regeneration; Inflammation; Apoptosis

期刊名称：JOURNAL OF FUNCTIONAL FOODS （ 影响因子：4.451； 五年影响因子：4.908 ）

ISSN： 1756-4646

年卷期： 2021 年 86 卷

页码：

收录情况： SCI

摘要： Cannabidiol (CBD) extracts have gained attention for their therapeutic efficacy. The present study used a zebrafish embryo caudal fin amputation model and found 1.25 mg/L crude CBD extract accelerated caudal fin regeneration and reduced apoptosis after amputation, while not altering mitotic regenerating cells. The purity of the CBD extract was 55.5 +/- 0.37%. Pure CBD promoted fin regeneration and inhibited neutrophil accumulation in a dose-dependent manner, while these effects were not seen in a high concentration of CBD (2.5 mg/L), possibly due to the toxicity of the high dose. An equivalent amount of pure CBD (0.625 to 1.25 mg/L) enhanced regeneration and reduced apoptosis similarly to crude CBD extract (1.25 mg/L). Additionally, CBD reduced the gene expression level of cxcl8, tnf-a, and il-1 beta, and protein expression level of IL-1 beta, Caspase 3, and PARP, but not Bcl-2 or Bax. These results suggest that CBD exerts its effect on wound pro-regeneration in part by modulating the inflammatory response and the IL-1 beta/Caspase 3/PARP signal pathway.