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Knockdown of the ecdysone receptor disrupts development and causes mortality in the melon fly, Zeugodacus cucurbitae

文献类型： 外文期刊

作者： Ahmad, Shakil 1 ; Jamil, Momana 1 ; Jaworski, Coline C. 2 ; Wu, Yuejie 1 ; Palma-Onetto, Valeria 3 ; Lyu, Baoqian 4 ; Luo, Yanping 1 ;

作者机构： 1.Hainan Univ, Sch Plant Protect, Dept Pesticide Sci, Haikou, Hainan, Peoples R China

2.Univ Cote Azur, INRAE, UMR ISA, Nice, France

3.Univ Catolica Santisima Concepcion, Fac Ciencias, Dept Quim Ambiental, Concepcion, Chile

4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Key Lab Integrated Pest Management Trop Crops, Minist Agr & Rural Affairs, Haikou, Peoples R China

5.Hainan Univ, Sch Plant Protect, Haikou 570228, Hainan, Peoples R China

关键词： ecdysone receptor; gene silencing; lethal and sublethal effects; oral delivery; RNA interference

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：2.6； 五年影响因子：2.9 ）

ISSN： 0962-1075

年卷期： 2023 年 32 卷 6 期

页码：

收录情况： SCI

摘要： Cucurbits are important economic plants that are attacked by numerous pests, among which the melon fly Zeugodacus cucurbitae is extremely problematic. New sustainable pest control strategies are necessary to replace chemical insecticides that are harmful to the environment, human health and nontarget species. The RNA interference (RNAi) technology is one of the most promising tools due to high efficiency and species specificity. We developed an RNAi strategy targeting the ecdysone receptor (ECR) of Z. cucurbitae, which plays an important role in moulting and reproduction. We identified, described and isolated the ECR gene of Z. cucurbitae and measured its expression pattern across developmental stages and tissues. ZcECR knockdown via dsZcECR ingestion caused a significant larval mortality and abnormal phenotypes in pupae and adults. About 68% of larvae fed with a dsZcECR-treated diet failed to enter the pupal stage and died. In addition, ZcECR knockdown dramatically reduced pupal weight (by 3.24 mg on average) and fecundity (by about 23%). RNAi targeting the ECR gene is therefore a promising method to control Z. cucurbitae, paving the way for the development of novel sustainable and highly specific control strategies.

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