Light controls mesophyll- specific posttranscriptional splicing of photoregulatory genes by AtPRMT5
文献类型： 外文期刊
作者： Yan, Yan 1 ; Luo, Haofei 1 ; Qin, Yuwei 3 ; Yan, Tingting 4 ; Jia, Jinbu 3 ; Hou, Yifeng 1 ; Liu, Zhijian 3 ; Zhai, Jixian 3 ; Long, Yanping 3 ; Deng, Xian 1 ; Cao, Xiaofeng 1 ;
作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Seed Innovat, State Key Lab Plant Genom, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China
3.Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen 518055, Peoples R China
4.Hainan Acad Agr Sci, Inst Trop Fruit Trees, Key Lab Trop Fruit Tree Biol Hainan Prov, Haikou 571100, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
关键词： post transcriptional splicing; single- nucleus RNA profiling; mesophyll cell; AtPRMT5; COP1
期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.1； 五年影响因子：12.0 ）
ISSN： 0027-8424
年卷期： 2024 年 121 卷 6 期
页码：
收录情况： SCI
摘要： Light plays a central role in plant growth and development, providing an energy source and governing various aspects of plant morphology. Previous study showed that many polyadenylated full- length RNA molecules within the nucleus contain unspliced introns (post- transcriptionally spliced introns, PTS introns), which may play a role in rapidly responding to changes in environmental signals. However, the mechanism underlying post- transcriptional regulation during initial light exposure of young, etiolated seedlings remains elusive. In this study, we used FLEP-seq2, a Nanopore-based sequencing technique, to analyze nuclear RNAs in Arabidopsis (Arabidopsis thaliana) seedlings under different light conditions and found numerous light- responsive PTS introns. We also used single- nucleus RNA sequencing (snRNA-seq) to profile transcripts in single nucleus and investigate the distribution of light- responsive PTS introns across distinct cell types. We established that light- induced PTS introns are predominant in mesophyll cells during seedling de- etiolation following exposure of etiolated seedlings to light. We further demonstrated the involvement of the splicing- related factor A. thaliana PROTEIN ARGININE METHYLTRANSFERASE 5 (AtPRMT5), working in concert with the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), a critical repressor of light signaling pathways. We showed that these two proteins orchestrate light- induced PTS events in mesophyll cells and facilitate chloroplast development, photosynthesis, and morphogenesis in response to ever- changing light conditions. These findings provide crucial insights into the intricate mechanisms underlying plant acclimation to light at the cell - type level.
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