Potential Misidentification of Natural Isomers and Mass-Analogs of Modified Nucleosides by Liquid Chromatography-Triple Quadrupole Mass Spectrometry
文献类型： 外文期刊
作者： Lin, Xiuying 1 ; Zhang, Qianhui 2 ; Qin, Yichao 1 ; Zhong, Qisheng 3 ; Lv, Daizhu 4 ; Wu, Xiaopeng 4 ; Fu, Pengcheng 1 ; Lin, Huan 1 ;
作者机构： 1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China
2.Hainan Univ, Coll Food Sci & Engn, Haikou 570228, Hainan, Peoples R China
3.Guangzhou Branch, Shimadzu China Corp, Guangzhou 510656, Peoples R China
4.Chinese Acad Trop Agr Sci, Anal & Testing Ctr, Haikou 571101, Hainan, Peoples R China
关键词： nucleoside misidentification; RNA modification; epitranscriptomics; LC-MS; MS; tRNA; HILIC
期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）
ISSN：
年卷期： 2022 年 13 卷 5 期
页码：
收录情况： SCI
摘要： Triple quadrupole mass spectrometry coupled to liquid chromatography (LC-TQ-MS) can detect and quantify modified nucleosides present in various types of RNA, and is being used increasingly in epitranscriptomics. However, due to the low resolution of TQ-MS and the structural complexity of the many naturally modified nucleosides identified to date (>160), the discrimination of isomers and mass-analogs can be problematic and is often overlooked. This study analyzes 17 nucleoside standards by LC-TQ-MS with separation on three different analytical columns and discusses, with examples, three major causes of analyte misidentification: structural isomers, mass-analogs, and isotopic crosstalk. It is hoped that this overview and practical examples will help to strengthen the accuracy of the identification of modified nucleosides by LC-TQ-MS.
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