Chromosome-level genome assembly of the largefin longbarbel catfish (Hemibagrus macropterus)
文献类型： 外文期刊
作者： Ye, Huan 1 ; Fan, Jiahui 1 ; Hou, Yanling 1 ; Yue, Huamei 1 ; Ruan, Rui 1 ; Li, Shuang 2 ; Hu, Chongjiang 2 ; Xie, Yong 2 ; Li, Chuangju 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China
2.Chongqing Fishery Sci Res Inst, Chongqing, Peoples R China
关键词： Hemibagrus macropterus; genome assembly; genome annotation; comparative genomics; Hi-C; PacBio
期刊名称：FRONTIERS IN GENETICS （ 影响因子：3.7； 五年影响因子：4.3 ）
ISSN：
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： The largefin longbarbel catfish, Hemibagrus macropterus, is an economically important fish species in southwestern China, with males growing faster than females. This study presents a high-quality chromosome-level genome assembly of the largefin longbarbel catfish, generated by integrating Illumina short reads, PacBio HiFi long reads, and Hi-C data. The assembled genome size was 858.5 Mb, with a contig and scaffold N50 of 5.8 Mb and 28.4 Mb, respectively. A total of 656 contigs were successfully anchored to 30 pseudochromosomes with a BUSCO score of 97.7%, consistent with the number of chromosomes analyzed by karyotype. The genome contained 29.5% repeat sequences, and a predicted total of 26,613 protein-coding genes, of which 25,769 (96.8%) were functionally annotated in differen databases. Evolutionary analysis showed that H. macropterus was most closely related to H. wyckioides, with a divergence time of approximately 16.3 million years. Chromosomal syntenic relationships among H. macropterus, H. wyckioides, and Pelteobagrus fulvidraco revealed a one-to-one relationship for most chromosomes, except for break, fission, and inversion of some chromosomes. The first high-quality reference genome will not only provide a valuable genetic resource for the study of sex determination mechanisms and genetic breeding of largefin longbarbel catfish, but also contribute to comparative analyses of genome and chromosome evolution within Siluriformes.
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