文献类型： 外文期刊
作者： Wu, Xiaohui 1 ; Zhang, Mengyuan 4 ; Zheng, Zheng 2 ; Sun, Ziqi 2 ; Qi, Feiyan 2 ; Liu, Hua 2 ; Wang, Juan 2 ; Wang, Mengmeng 2 ; Zhao, Ruifang 2 ; Wu, Yue 2 ; Wang, Xiao 2 ; Liu, Hongfei 2 ; Dong, Wenzhao 2 ; Zhang, Xinyou 2 ;
作者机构： 1.Henan Agr Univ, Coll Agron, Zhengzhou 450046, Peoples R China
2.Minist Agr & Rural Affairs, Henan Prov Key Lab Oil Crops Improvement, Key Lab Oil Crops Huang Huai Hai Plains, Henan Acad Agr Sci,Henan Acad Crop Mol Breeding, Zhengzhou 450002, Peoples R China
3.Shennong Lab, Zhengzhou 450002, Peoples R China
4.Shangqiu Acad Agr & Forestry Sci, Shangqiu 476000, Peoples R China
关键词： peanut web blotch; bulked segregant analysis; KASP markers; resistant gene
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）
ISSN： 2095-3119
年卷期： 2024 年 23 卷 5 期
页码：
收录情况： SCI
摘要： Peanut (Arachis hypogaea L.) is a globally important oil crop. Web blotch is one of the most important foliar diseases affecting peanut, which results in serious yield losses worldwide. Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch. In the current study, a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch resistance. Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR (KASP) markers, we identified a novel web blotch resistancerelated locus spanning approximately 169 kb on chromosome 16. This region included four annotated genes, of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents. Two markers (Chr.16.12872635 and Chr.16.12966357) linked to this gene were shown to be cosegregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines (RIL), which could be used in marker-assisted breeding of resistant peanut varieties.
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