文献类型： 外文期刊
作者： Gao, Feng-ying 1 ; Qu, Lan 1 ; Yu, Shao-guo; Ye, Xing 1 ; Tian, Yuan-yuan 1 ; Zhang, Li-li 1 ; Bai, Jun-jie 1 ; Lu, Maixin 1 ;
作者机构： 1.Minist Agr, Key Lab Trop & Subtrop Fisheries Resource Utiliza, Guangzhou 510380, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 20130
关键词： Oreochromis aureus; C-type lysozyme; Bacterial challenge; Prokaryotic expression; Lytic activities
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2012 年 32 卷 5 期
页码：
收录情况： SCI
摘要： Lysozyme is an important molecule of innate immune system for the defense against bacterial infections. Three genes encoding chicken-type (c-type) lysozymes, C1-, C2-, C3-type, were obtained from tilapia Oreochromis aureus by RT-PCR and the RACE method. Catalytic and other conserved structure residues required for functionality were identified. The amino acid sequence identities between C1- and C2-type, C1- and C3-type, C2- and C3-type were 67.8%, 65.7% and 63.9%, respectively. Phylogenetic tree analyze indicated the three genes were firstly grouped to those of higher teleosteans, Pleuronectiformes and Tetraodontiformes fishes, and then clustered to those of lower teleosteans, Cypriniformes fishes. Bioinformatic analysis of mature peptide showed that the three genes possess typical sequence characteristics, secondary and tertiary structure of c-type lysozymes. The three tilapia c-type lysozymes mRNAs were mainly expressed in liver and muscle, and C1-type lysozyme also highly expressed in intestine. cl type lysozyme mRNA was weakly expressed in stomach, C2- and C3-type mRNAs were weakly expressed in intestine. After bacterial challenge, up-regulation was obvious in kidney and spleen for C1-type lysozyme mRNA, while for C2- and C3-type lysozyme obvious increase were observed in stomach and liver, suggesting that C1-type lysozyme may mainly play roles in defense, while C2- and C3-type lysozyme mainly conduct digestive function against bacteria infection. All the three c-type recombinant lysozymes displayed lyric activity against Gram-negative and Gram-positive bacteria. These results indicated that three c-type lysozymes play important roles in the defense of O. aureus against bacteria infections. (C) 2012 Elsevier Ltd. All rights reserved.
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