High-throughput sequencing identification and multiplex RT-PCR detection of pepper viruses
文献类型： 外文期刊
作者： Luo, Chun 1 ; Luo, Yao 1 ; Luo, Hao 1 ; Han, Ke 1 ; Lei, Lei 2 ; Jia, Meng-ao 3 ; Xu, Tengzhi 1 ;
作者机构： 1.Guizhou Univ, Inst Crop Protect, Coll Agr, Guiyang 550025, Peoples R China
2.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang 550008, Peoples R China
3.Guizhou Acad Tobacco Sci, Guiyang 550001, Guizhou, Peoples R China
关键词： Pepper virus; High-throughput sequencing; Synergistic infection; Multiplex RT-PCR; Virus detection
期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：3.3； 五年影响因子：3.2 ）
ISSN： 0885-5765
年卷期： 2025 年 136 卷
页码：
收录情况： SCI
摘要： Peppers are susceptible to a variety of virus infections. Early and accurate detection of virus species is the prerequisite for the virus disease prevention and management to reduce yield and quality losses. This study utilized small RNA sequencing to investigate the viruses in Guizhou peppers. Through extensive bioinformatics analysis, we identified ten virus species infecting Guizhou peppers and obtained the sequences of the conserved protein encoded by these viruses. Our phylogenetic analyses clarified the taxonomic status of the obtained viral isolate. A rapid and cost-effective multiplex PCR method for simultaneous virus detection was developed. By testing the novel developed method using field samples, we found that CMV, PCV1 and BBWV2 were the three most common viruses. This research offers a comprehensive list of viruses affecting peppers in Guizhou and presents an efficient detection method, facilitating our understanding and practical management of pepper virus infections.
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