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Using high-throughput multiple optical phenotyping to decipher the genetic architecture of maize drought tolerance

文献类型： 外文期刊

作者： Wu, Xi 1 ; Feng, Hui 1 ; Wu, Di 1 ; Yan, Shijuan 4 ; Zhang, Pei 1 ; Wang, Wenbin 1 ; Zhang, Jun 1 ; Ye, Junli 1 ; Dai, Guoxi 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Natl Ctr Plant Gene Res, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Hubei Key Lab Agr Bioinformat, Wuhan 430070, Peoples R China

3.Hubei Hongshan Lab, Wuhan 430070, Peoples R China

4.Guangdong Acad Agr Sci, Guangzhou 510640, Peoples R China

5.Cornell Univ, Sch Integrat Plant Sci, Sect Plant Breeding & Genet, Ithaca, NY 14850 USA

6.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China

7.Wilkes Univ, Dept Biol, Wilkes Barre, PA 18766 USA

8.Cornell Univ, Inst Genom Divers, Ithaca, NY 14850 USA

9.ARS, USDA, Ithaca, NY 14850 USA

期刊名称：GENOME BIOLOGY （ 影响因子：13.583； 五年影响因子：17.433 ）

ISSN： 1474-760X

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Drought threatens the food supply of the world population. Dissecting the dynamic responses of plants to drought will be beneficial for breeding drought-tolerant crops, as the genetic controls of these responses remain largely unknown. Results Here we develop a high-throughput multiple optical phenotyping system to noninvasively phenotype 368 maize genotypes with or without drought stress over a course of 98 days, and collected multiple optical images, including color camera scanning, hyperspectral imaging, and X-ray computed tomography images. We develop high-throughput analysis pipelines to extract image-based traits (i-traits). Of these i-traits, 10,080 were effective and heritable indicators of maize external and internal drought responses. An i-trait-based genome-wide association study reveals 4322 significant locus-trait associations, representing 1529 quantitative trait loci (QTLs) and 2318 candidate genes, many that co-localize with previously reported maize drought responsive QTLs. Expression QTL (eQTL) analysis uncovers many local and distant regulatory variants that control the expression of the candidate genes. We use genetic mutation analysis to validate two new genes, ZmcPGM2 and ZmFAB1A, which regulate i-traits and drought tolerance. Moreover, the value of the candidate genes as drought-tolerant genetic markers is revealed by genome selection analysis, and 15 i-traits are identified as potential markers for maize drought tolerance breeding. Conclusion Our study demonstrates that combining high-throughput multiple optical phenotyping and GWAS is a novel and effective approach to dissect the genetic architecture of complex traits and clone drought-tolerance associated genes.

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