文献类型： 外文期刊
作者： Meng, Fanzeng 1 ; Wang, Yiting 2 ; Wang, Guohao 1 ; Hu, Tao 3 ; Xu, La 4 ; Tang, Kathy F. J. 2 ; Shi, Weifeng 3 ; Zhang, Fan 2 ; Dong, Xuan 2 ; Huang, Jie 1 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst,Qingdao Key Lab Mar, Lab Marine Fisheries Sci & Food Prod Proc,Minist, Pilot Natl Lab Marine Sci & Technol Qingdao,Key L, Qingdao 266071, Peoples R China
3.Shandong First Med Univ Shandong Acad Med Sci, Sch Publ Hlth, Tai An 271000, Shandong, Peoples R China
4.Shandong Key Lab Dis Control Mariculture, Qingdao 266104, Peoples R China
5.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
6.Network Aquaculture Ctr Asia Pacific, Bangkok 10090, Thailand
关键词： Macrobrachium rosenbergii; Macrobrachium rosenbergii golda virus; Goldavirus; Nidovirales; aquaculture
期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）
ISSN： 2076-2615
年卷期： 2022 年 12 卷 1 期
页码：
收录情况： SCI
摘要： Simple Summary Macrobrachium rosenbergii golda virus (MrGV) was first identified in Macrobrachium rosenbergii in Bangladesh with massive larva death. In this study, the variant of MrGV Mr-18 from China was accidentally found in a meta-transcriptome study of M. rosenbergii and compared with MrGV LH1-2018 reported in Bangladesh. Phylogenetic analysis has shown that these two variants belong to the same, yet unclassified, genus. At present, there has no evidence that MrGV Mr-18 causes disease in M. rosenbergii. However, we should be alert that MrGV may lead to the mass death of M. rosenbergii larvae; thus, surveillance of MrGV in Asia should be given priority. In a meta-transcriptome study of the giant freshwater prawn Macrobrachium rosenbergii sampled in 2018 from a hatchery, we identified a variant of Macrobrachium rosenbergii golda virus (MrGV) in postlarvae without clinical signs. The virus belongs to the family Roniviridae, and the genome of this MrGV variant, Mr-18, consisted of 28,957 nucleotides, including 4 open reading frames (ORFs): (1) ORF1a, encoding a 3C-like protein (3CLP) (4933 aa); (2) ORF1b, encoding a replicase polyprotein (2877 aa); (3) ORF2, encoding a hypothetical nucleocapsid protein (125 aa); and (4) ORF3, encoding a glycoprotein (1503 aa). ORF1a overlaps with ORF1b with 40 nucleotides, where a -1 ribosomal frameshift with slippage sequence 5 '-G(14925)GGUUUU(14931)-3 ' produces the pp1ab polyprotein. The genomic sequence of Mr-18 shared 97.80% identity with MrGV LH1-2018 discovered in Bangladesh. The amino acid sequence identities between them were 99.30% (ORF1a), 99.60% (ORF1b), 100.00% (ORF2), and 99.80% (ORF3), respectively. Phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) proteins revealed that they clustered together and formed a separate cluster from the genus Okavirus. The finding of MrGV in China warrants further studies to determine its pathogenicity and prevalence within the region.
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