Transcriptomics and metabolomics reveal the molecular and metabolic adaptation to heat stress in Manila clam Ruditapes philippinarum
文献类型： 外文期刊
作者： Jing, Hao 1 ; Zhou, Liqing 1 ; Gao, Yiqiang 1 ; Liu, Zhihong 1 ; Wu, Biao 1 ; Sun, Xiujun 1 ; Tu, Kang 5 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao, Peoples R China
2.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
4.Jiangsu Ocean Univ, Sch Marine Sci & Fisheries, Lianyungang, Peoples R China
5.Putian Inst Aquaculture Sci Fujian Prov, Seawater Res Dept, Putian, Peoples R China
关键词： heat stress; Ruditapes philippinarum; transcriptomic; metabolomic; enzymatic activity
期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：3.7； 五年影响因子：4.7 ）
ISSN：
年卷期： 2023 年 10 卷
页码：
收录情况： SCI
摘要： Temperature is an important environmental factor affecting metabolism, growth and life activities of marine bivalves. To reveal the molecular and metabolic responses to heat stress, comparative transcriptomics and metabolomics were performed in the commercially important bivalve, Manila clam Ruditapes philippinarum. Comparative transcriptomics revealed a total of 3980 differential genes (DEGs) mainly involved in energy metabolism, protein processing, glycerolphospholipid metabolism, inoxidizability and anti-apoptosis. Comparative metabolomic analysis identified 293 differential metabolites (DEMs) in the heat-stressed clams, including Acetyl-CoA, Beta-D-Glucose, phosphatidylcholine and free amino acids. For the heat-stress clams, they showed the significantly increased enzyme activities of AKP and ACP, as well as antioxidant-related enzyme activities (SOD and CAT) and oxidative damage substance (MDA). The changes in gene expression of glycolysis-related genes (GLUT1, GLK, HK1 and GPI6) and metabolites (Beta-D-Glucose and Acetyl-CoA) reflect the adjusted energy metabolism in the heat-stressed clams. The protein processing in endoplasmic reticulum may play the important roles in the stability of cell membrane structure during heat adaptation. The increased contents of glycerophospholipid (PC) may be essential to maintain the stability and permeability of cell membrane in the heat-stressed clams. Molecular chaperones (HSP70 and HSP90) may participate in the degradation of misfolded proteins to maintain cell homeostasis during the heat stress. To defense the heat stress, clams also initiate the protection and defense mechanisms by activating antioxidant system, innate immune system and anti-apoptotic system. These findings will shed new lights on molecular and metabolic adaptation to heat stress in the intertidal mollusks in a warming climate at the global scale.
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