Orthologous genes Pm12 and Pm21 from two wild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance
文献类型： 外文期刊
作者： Zhu, Shanying 1 ; Liu, Cheng 3 ; Gong, Shuangjun 4 ; Chen, Zhaozhao 2 ; Chen, Rong 5 ; Liu, Tianlei 2 ; Liu, Renkang 2 ; Du, Haonan 2 ; Guo, Rui 2 ; Li, Genying 3 ; Li, Miaomiao 6 ; Fan, Renchun 6 ; Liu, Zhiyong 6 ; Shen, Qian-Hua 6 ; Gao, Anli 5 ; Ma, Pengtao 7 ; He, Huagang 2 ;
作者机构： 1.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China
2.Jiangsu Univ, Sch Life Sci, Zhenjiang 212013, Peoples R China
3.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Peoples R China
4.Hubei Acad Agr Sci, Inst Plant Protect & Soil Sci, Wuhan 430064, Peoples R China
5.Henan Univ, Sch Life Sci, Kaifeng 475004, Peoples R China
6.Chinese Acad Sci, Inst Genet & Dev Biol, Innovat Acad Seed Design, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
7.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China
关键词： Pm12; Pm21; powdery mildew resistance; evolutionary conservation; race specificity; intramolecular interaction
期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）
ISSN： 2590-3462
年卷期： 2023 年 4 卷 2 期
页码：
收录情况： SCI
摘要： Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that threatens wheat production worldwide. Pm12, which originated from Aegilops speltoides, a wild relative of wheat, confers strong resistance to powdery mildew and therefore has potential use in wheat breeding. Using susceptible mutants induced by gamma irradiation, we physically mapped and isolated Pm12 and showed it to be orthologous to Pm21 from Dasypyrum villosum, also a wild relative of wheat. The resistance function of Pm12 was validated via ethyl methanesulfonate mutagenesis, virus-induced gene silencing, and stable genetic transformation. Evolutionary analysis indicates that the Pm12/Pm21 loci in wheat species are relatively conserved but dynamic. Here, we demonstrated that the two orthologous genes, Pm12 and Pm21, possess differential resistance against the same set of Bgt isolates. Overexpression of the coiled-coil domains of both PM12 and PM21 induces cell death in Nicotiana benthamiana leaves. However, their full-length forms display different cell death-inducing activities caused by their distinct intramolecular in-teractions. Cloning of Pm12 will facilitate its application in wheat breeding programs. This study also gives new insight into two orthologous resistance genes, Pm12 and Pm21, which show different race specificities and intramolecular interaction patterns.
- 相关文献
作者其他论文 更多>>
-
The wheat NLR pair RXL/Pm5e confers resistance to powdery mildew
作者：Guo, Guanghao;Hou, Yikun;Zhang, Huaizhi;Lu, Ping;Li, Miaomiao;Dong, Lingli;Xie, Jingzhong;Zhu, Keyu;Li, Beibei;Dong, Lei;Yang, Yijun;Qiu, Dan;Wang, Gaojie;Zhao, Yusheng;Chen, Yuhang;Liu, Zhiyong;Guo, Guanghao;Ahn, Hee-Kyung;Zhao, He;Jones, Jonathan D. G.;Bai, Kaihong;Hou, Yikun;Li, Beibei;Dong, Lei;Liu, Zhiyong;Gong, Zhen;Han, Guan-Zhu;Wu, Qiuhong;Chen, Yongxing;Li, Wenling;Li, Hongjie;Zhang, Panpan;Yuan, Chengguo;Shi, Wenqi;Xue, Minfeng;Yang, Lijun;Yu, Dazao;Hu, Tiezhu;Liu, Zhiyong;Ahn, Hee-Kyung
关键词：wheat; powdery mildew; NLR pair; hetero-complexes; resistance mechanism
-
Identification of SepF in Streptococcus suis involving cell division
作者：Gao, Ting;Zhu, Jiajia;Liu, Wei;Yang, Keli;Zhang, Tengfei;Yuan, Fangyan;Liu, Zewen;Guo, Rui;Li, Chang;Wu, Qiong;Tian, Yongxiang;Zhou, Danna;Li, Tingting;Zheng, Linlin;Zhou, Rui;Zheng, Linlin;Chen, Mo
关键词：
Streptococcus suis ; Cell division; Septum; Protein-protein interaction; Drug target -
The putative effector BgtE-20069a, secreted from Blumeria graminis f. sp. tritici, suppresses plant immunity to facilitate wheat infection
作者：Yan, Tong;Cheng, Gui;Zhai, Yamei;Wu, Lisha;Gong, Shuangjun;Yu, Dazhao;Yang, Lijun;Zeng, Fansong;Cheng, Gui;Zhai, Yamei;Wu, Lisha
关键词：Blumeria graminis f. sp. tritici; Effector; Virulence; Plant immunity
-
Antibacterial activity of a plant natural poly-phenol against zoonotic Streptococcus suis
作者：Gao, Ting;Wang, Yanjun;Zhu, Jiajia;Chen, Mo;Yang, Keli;Yuan, Fangyan;Liu, Zewen;Liu, Wei;Guo, Rui;Li, Chang;Wu, Qiong;Tian, Yongxiang;Zhou, Danna;Wang, Yanjun;Li, Lu;Chen, Mo;Tian, Haibin
关键词：Streptococcus suis; Plant resource; Poly-phenol; Biofilm; Antibiotic alternative; Antibiotic adjuvant
-
Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew
作者：Fu, Bisheng;Zhang, Qiaofeng;Liu, Caiyun;Cai, Jin;Guo, Wei;Liu, Ying;Zhai, Wenling;Wu, Jizhong;Fu, Bisheng;Liu, Caiyun;Cai, Jin;Guo, Wei;Wu, Jizhong;Fu, Bisheng;Wu, Jizhong;Lin, Zhixin;Xu, Feng;Yan, Lijuan;Gong, Shuangjun
关键词：
Blumeria graminis ; genetic markers; resistance breeding;Triticum aestivum L.;PmHHM -
Deletion of FgAtg27 decreases the pathogenicity of Fusarium graminearum through influence autophagic process
作者：Wen, Yong;Wang, Mengru;Liu, Xi;Yin, Xiaohui;Yin, Junliang;Gong, Shuangjun
关键词：Autophagy; FgAtg27; Fusarium graminearum
-
Prevalence, Molecular Characterization, and Antimicrobial Resistance Profile of Enterotoxigenic Escherichia coli Isolates from Pig Farms in China
作者：Zhu, Jiajia;Liu, Zewen;Wang, Siyi;Gao, Ting;Liu, Wei;Yang, Keli;Yuan, Fangyan;Wu, Qiong;Li, Chang;Guo, Rui;Tian, Yongxiang;Zhou, Danna;Wang, Siyi
关键词：Enterotoxigenic
Escherichia coli ; MLST; serotypes; virulence genes; minimum inhibitory concentration; antimicrobial resistance genes
