Transcriptome and Metabolome Analyses Reveal Mechanisms Underlying the Response of Quinoa Seedlings to Nitrogen Fertilizers
文献类型: 外文期刊
作者: Li, Hanxue 1 ; Wang, Qianchao 1 ; Huang, Tingzhi 1 ; Liu, Junna 1 ; Zhang, Ping 1 ; Li, Li 1 ; Xie, Heng 1 ; Wang, Hongxin 1 ; Liu, Chenghong 2 ; Qin, Peng 1 ;
作者机构: 1.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Peoples R China
2.Shanghai Acad Agr Sci, Biotech Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
关键词: quinoa seeding; nitrogen; metabolomics; transcriptomics
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.6; 五年影响因子:6.2 )
ISSN: 1661-6596
年卷期: 2023 年 24 卷 14 期
页码:
收录情况: SCI
摘要: Quinoa (Chenopodium quinoa Willd.) is a dicotyledonous annual amaranth herb that belongs to the family Chenopodiaceae. Quinoa can be cultivated across a wide range of climatic conditions. With regard to its cultivation, nitrogen-based fertilizers have a demonstrable effect on the growth and development of quinoa. How crops respond to the application of nitrogen affects grain quality and yield. Therefore, to explore the regulatory mechanisms that underlie the responses of quinoa seedlings to the application of nitrogen, we selected two varieties (i.e., Dianli-1299 and Dianli-71) of quinoa seedlings and analyzed them using metabolomic and transcriptomic techniques. Specifically, we studied the mechanisms underlying the responses of quinoa seedlings to varying concentrations of nitrogen by analyzing the dynamics of metabolites and genes involved in arginine biosynthesis; carbon fixation; and alanine, aspartate, and glutamate biosynthetic pathways. Overall, we found that differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) of quinoa are affected by the concentration of nitrogen. We detected 1057 metabolites, and 29,012 genes were annotated for the KEGG. We also found that 15 DEMs and 8 DEGs were key determinants of the differences observed in quinoa seedlings under different nitrogen concentrations. These contribute toward a deeper understanding of the metabolic processes of plants under different nitrogen treatments and provide a theoretical basis for improving the nitrogen use efficiency (NUE) of quinoa.
- 相关文献
作者其他论文 更多>>
-
Integration of transcriptome and metabolome reveals the accumulation of related metabolites and gene regulation networks during quinoa seed development
作者:Wang, Qianchao;Liu, Junna;Zhang, Ping;Li, Li;Xie, Heng;Li, Hanxue;Wang, Hongxin;Qin, Peng;Shi, Jirong;Liu, Chenghong
关键词:Quinoa seeds,; Development period,; Transcriptome,; Metabolic group,; Regulatory network
-
Key phytochemicals contributing to the bitterness of quinoa
作者:Guo, Huimin;Liu, Chenghong;Xu, Hongwei;Wang, Siyu;Bao, Yuying;Ren, Guixing;Ren, Guixing;Bao, Yuying;Ren, Guixing;Yang, Xiushi
关键词:Triterpenoid; Kaempferol derivative; Bitterness; Sensory evaluation; Dose -over -threshold
-
Genetic Diversity and Genome-Wide Association Analysis of the Hulled/Naked Trait in a Barley Collection from Shanghai Agricultural Gene Bank
作者:Chen, Zhiwei;Guo, Zhenzhu;Li, Luli;Guo, Guimei;Zhang, Shuwei;Zong, Yingjie;Liu, Shiseng;Liu, Chenghong;Zhou, Longhua;Halford, Nigel G.
关键词:Hordeum vulgare L.; naked trait; genotyping-by-sequencing (GBS); genome-wide association studies (GWAS); Kompetitive Allele Specific PCR (KASP)
-
Efficient isolated microspore culture protocol for callus induction and plantlet regeneration in japonica rice (Oryza sativa L.)
作者:Gao, Runhong;Zong, Yingjie;Zhang, Shuwei;Guo, Guimei;Zhang, Wenqi;Chen, Zhiwei;Lu, Ruiju;Liu, Chenghong;Wang, Yifei;Li, Yingbo
关键词:Isolated microspore culture; Oryza sativa L.; Low-temperature pre-treatment; Microspore developmental stage; Callus induction; Plantlet regeneration
-
Advances in Identifying the Mechanisms by Which Microorganisms Improve Barley Salt Tolerance
作者:Chen, Zhiwei;Yan, Xin;Chen, Zhiwei;Guo, Zhenzhu;Zhou, Longhua;Xu, Hongwei;Liu, Chenghong
关键词:Hordeum vulgare L.; microbiome; salt tolerance; omics; bacteria community
-
Characterization of the aroma in barley leaves from different cultivars and tillering stages by HS-SPME/GC-MS, GC-O and E-nose
作者:Huang, Juan;Yang, Mengfei;Yu, Haiyan;Chen, Chen;Tian, Huaixiang;Zong, Yingjie;Liu, Chenghong
关键词:Barley leaf; Gas chromatography -mass spectrometry; Gas chromatography-olfactometry; Quantitative descriptive analysis; E -nose; Multivariate statistical analysis
-
Transcriptomics integrated with metabolomics reveals the effect of cold stress on rice microspores
作者:Li, Yingbo;Zong, Yingjie;Li, Wenrui;Guo, Guimei;Zhou, Longhua;Xu, Hongwei;Gao, Runhong;Liu, Chenghong;Li, Yingbo;Zong, Yingjie;Li, Wenrui;Guo, Guimei;Zhou, Longhua;Xu, Hongwei;Gao, Runhong;Liu, Chenghong
关键词:Microspore embryogenesis; Cold stress; Rice; Transcriptome; Metabolomics