文献类型： 会议论文

第一作者： ZHANG Dong-jie

作者： ZHANG Dong-jie ¹ ; LIU Di ¹ ;

作者机构： 1.HeiLongJiang Academy of Agricultural Sciences, HAAS

关键词： OPN;Phylogenetic tree;RT-PCR;Western-blot;Wild boar

会议名称： iCBBE 2009

主办单位：

页码： 800-803

摘要： The wild boar OPN cDNA in size of 909bp was cloned by RT-PCR and analyzed by bioinformatics. The result showed that wild boar OPN contains 1) a potential calcium phosphate apatite binding region of eight consecutive Asp residues beginning at amino acid 86, 2) a cell-attachment Gly-Arg-Gly-Asp-Ser (GRGDS) sequence (residues 153-157), 3) a thrombin RS cleavage site (residues 163 and 164). The phylogenetic tree showed that the relationship between wild boar and domestic pig was the most close, but farthest with zebrafish, OPN gene accord with evolution relation. Wild boar OPN mRNA was abroad expressed in different tissue, higher in heart, stomach, lung, muscle, uterus, and lower in liver, kidney, small intestine and ovary. The protein weight was different in different tissue 70kDa, 70kDa and 45kDa, 70kDa, 45kDa and 24kDa.

分类号： G202-53