文献类型： 会议论文

第一作者： Qiao Yang

作者： Qiao Yang ¹ ; Xiaoling Zhang ² ; Wenjun Zhang ³ ; Lei Zhang ¹ ; Jun Dai ¹ ; Chengxiang Fang ¹ ; Binghua Jiao ⁴ ;

作者机构： 1.College of Life Sciences, Wuhan University

2.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences

3.College of Food Sciences, Shanghai Ocean University

4.Department of Biochemistry and Molecular Biology, Second Military Medical University

关键词： Deinococcus radiodurans;Carotenoid;Biosynthesis genes

会议名称： iCBBE 2009

主办单位：

页码： 483-486

摘要： To investigate the biosynthetic genes and the biological functions of carotenoids in the radioresistant mechanisms of the extremely radioresistant bacterium, Deinococcus radiodurans, two colorless mutants knocking out of the phytoene synthase (crtB) and phytoene desaturase (crtI) genes were constructed by PCR and in vivo homologous recombination. The carotenoids of this bacterium were extracted and analyzed by HPLC-MS technique. Comparative analysis of the sensitivities among the two colorless mutants and wild R1 showed that the two colorless mutants were more sensitive to ionizing radiation (IR) and hydrogen peroxide, indicating that the deletion of crtB and crtI genes made the mutants unable to biosynthesize the crucial intermediate lycopene and series of downstreaming carotenoids. This study has provided a new and direct evidence to explore the biosynthesis genes of carotenoids and their biological functions for the radioresistant mechanisms in the extremely radioresistant bacterium D. radiodurans.

分类号： G202-53