文献类型： 会议论文

第一作者： Ruhong Zhou

作者： Ruhong Zhou ¹ ; Zhenchen Zhang; Qing Wu; Rongxiang Fang; Keqiang Mang; Yingchuan Tian; Guiling Wa;

作者机构： 1.Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

关键词： biosafety;field tests;genetically modified;plants;microorganisms

会议名称： Proceedings of the 3rd international symposium on the biosafety results of field tests of genetically modified plants and microorganisms,November 13-16,1994 Monterey,California,US.

主办单位：

页码： 49-56

摘要： Coat protein (CP)-mediated resistance is one of the well-developed strategies in genetic engineering of plants against virus diseases. Transgenic plants expressing CP genes of tobacco mosaic virus (TMV) or cucumber mosaic virus (CMV) have been shownto be resistant to infection of the respective virus (Powell-Abel et al. 1986; Cuozzo et al. 1988). TMV and CMV are two major viral pathogens affecting tobacco plants in North China. We have thus developed transgenic tobacco plants resistant to infection of both TMV and CMV by simultaneously expressing CP genes of these two viruses in plants (Fang et al. 1990 ). Virus-challenge experiments conducted in fields and molecular analyses of expression of TMV and CMV CP genes were then performed to select virusresistant transgenic homozygous plant lines from the progeny of the primary transformants (Fang et al. 1993). In this paper we report a series of field trials on the progeny of the selected homozygous lines to verify the inheritance of the virus-resistance traits and to examine the quality of transgenic tobacco plants. We also report a large-scale field test using the homozygous line N364, showing significant protection of tobacco plants against virus infection.

分类号： Q