Effect of heterogeneous sperm and hybridization of DNA fragment in allogynogenetic silver crucian carp
文献类型： 会议论文
第一作者： XIA DEQUAN
作者： XIA DEQUAN 1 ; XUE GUOXIONG; ZHANG LI;
作者机构： 1.Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 Jiangsu, China
关键词： gene;DNA;RNA;enzymes;proteins
会议名称： Gene families:
主办单位：
页码： 251-262
摘要： The Fang Zheng silver crucian carp is a kind of bisexual triploid fish which can bring forth gynogenesis under induction of allogynogenetic fish sperm. The F1 generation of it, obtained under induction of sperm of wild carp, is called allogynogenetic silver crucian carp. The event that the heterogeneous fish sperm can also bring forth numerous biological effects in the filial generation, as it induces gynogenesis in fish, is called effect of heterogeneous sperm (heterogeneous sperm effect). RAPD was applied with 154 random primers in analyses of polymorphism in population of Fang Zheng silver crucian carp (female), wild carp (male) and their filial generation, allogynogenetic silver crucian carp. The electrophoretic bands related to of heterogeneous sperms effects were sought and used as probes after cloning, for Southern blot hybridization with the amplification products and genomes of above-mentioned tested fish. The results indicated that there exists a highly homogeneous fragment in the genomes of allogynogenetic crucian carp and wild carp, which is lacking in Fang Zheng silver crucian carp. It indicated the possible hybridization between the genome of the DNA fragments of male and female nuclei during fertilization. It provides a possible mechanism for the effect of heterogeneous sperm.
分类号： Q78
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