文献类型： 会议论文

第一作者： A. GASPAR

作者： A. GASPAR ¹ ; L. STRODIOT; PH. THONART;

作者机构： 1.Centre Wallon de Biologie Industrielle, Faculte universitaire des sciences agronomiques de Gembloux, 2, Passage Des Deportes, 8-5030 Belgium

关键词： xylanase;penicillium canescens;K_(1a);specific power

会议名称： Biotechnology for Fuels and Chemicals(19)

主办单位：

页码： 535-545

摘要： To improve xylanase productivity from Penicillium canescens 10-10c culture, an optimization of oxygen supply is required. Because the strain is sensitive to shear forces, leading to lower xylanase productivity as to morphological alteration, vigorous mixing is not desired. The influence of turbine design, agitation speed, and air flow rate on K_(1a) (global mass transfer coefficient, h~(-1)) and enzyme production is discussed. K_(1a) values increased with agitation speed and air flow rate, whatever the impeller, in our assay conditions. Agitation had more influence on K_(1a) values than air flow, when a disk-mounted blade's impeller (DT) is used; an opposite result was obtained with a hub-mounted pitched blade's impeller (PBT). Xylanase production appeared as a function of specific power (W/m~3), and an optimum was found in 20 and 100 L STRs fitted with DT impellers. On the other hand, the use of a hub-mounted pitched blade impeller (PBT8), instead of a disk-mounted blade impeller (DT4), reduced the lag time of hemicellulase production and increased xylanase productivity 1.3-fold.

分类号： Q5