文献类型： 会议论文
第一作者： K. Kouki
作者： K. Kouki 1 ; A. Mougou; R. Paul;
作者机构： 1.Faculte Universitaire des Sciences Agronomiques de Gembloux, Laboratoire de Toxicologie Environnementale, Passage des deportes, 2 B 5030 Gembloux, Belgique
关键词： growth;Lycopersicon;NaCl;root culture;tomato
会议名称： Developments in plant and soil sciences,,Vol.92.
主办单位：
页码： 428-429
摘要： In order to understand reactions of tomato roots towards NaCl stress when they are independent from photosynthetic organs, a stable and reliable technique for root culture was developed, It consisted of growing excised root tips of defined length ina shaken nutrient solution having a simple composition of Murashige and Skoog (MS) mineral nutrient, vitamin mix and sucrose, without the addition of any growth hormones. Special attention has been paid to standardise the culture technique because of the high sensitivity of root growth to environmental factors. In the first experiment, concentrations of 50 and 100 mM NaCl were used, subsequently, NaCl concentration was restricted to 75 mM, a single moderate stress level allowing the number of replications to be increased for more accurate statistical analysis. The roots of four L. esculentum Mill.cultivars, the ABA-deficient mutant flacca and ecotypes of Lycopersicon pennellii (corr.) d'Arcy, L. chilense Dunn. and L. cheesmanii f. minor (Hook f.) C. H. Mull. were successfully tested using the same technique.
分类号： Q945
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