文献类型: 会议论文
第一作者: Zhang Defu
作者: Zhang Defu 1 ; Karl Schellander;
作者机构: 1.Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences,Shanghai 201106
关键词: Pig;Nuclear transfer (NT);Activation method;Oocyte age
会议名称: Promoting global innovation of agricultural science & technology and sustainable agriculture development,Session 4
主办单位:
页码: 235-239
摘要: In this paper, we studied in vitro development of reconstituted porcine oocytes after nuclear transfer using cumulus ceUs (CC) and fibroblast cells (FC). Reconstituted oocytes by the fusion of CC showed the cleavage rate of 56.7%, developed to morula(11.7%) and hatching blastocysts (6.7%) which was higher than FC (p<0.05). This study also tested the effects of oocyte collection method, activation protocol and maturational age of recipient oocytes on the in vitro develpoment of nuclear transfer embryos reconstructed with cultured cumulus cells. Cumulus cells synchronized in GO/G1 phase by serum-starvation culture were transferred into enuclated oocytes that were collected by aspiration or dissection method and cultured for 33 or 44 h. Reconstituted embryos were activated with a combination of calcium ionophore A23187 or electric pulse and 6-DMAP, and cultured for 6 days. Oocytes collection methods, activation treatment in the presence of cytochalasin B and activation protocols did not affect the developmental rate of embryos reconstituted with 44-h-mature recipients. However, the development of embryos reconstituted with 33-h-mature recipients was significantly unproved (p<0.05) by activation with the combination of electric pulse and 6-DMAP. The present study show that reconstituted porcine embryos derived from cultured cumuluc cells can develop to the blastcyst stage, and that their development can be improved by reconstruction with young oocyte cytoplast following activation with a combinationof electric pulse and 6-DMAP.
分类号: S
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