文献类型： 会议论文

第一作者： Yan Zhao

作者： Yan Zhao ¹ ; Jin-feng Yun ² ; De-Fu Liu ² ;

作者机构： 1.College of Environment, Inner Mongolia Agricultural University, Huhhot, 010018, China Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Huhhot, 010031,China

2.College of Environment, Inner Mongolia Agricultural University, Huhhot, 010018, China

关键词： Agropyron mongolicum Keng;GSTs;Clone;Homology sequence

会议名称： Korea-China-Japan joint symposium on grassland agriculture and livestock production

主办单位：

页码： 134-135

摘要： In this study, GSTs gene homology fragment from Mongolian wheatgrass(Agropyron mongolicum Keng) was isolated using homologybased method, one partials gene sequences have been obtained by reverse transcription polymerase chain reaction (RT-PCR). sequence analysis using Software of DNAman and DNAuser etc, and showed that the cDNA sequences was 344 base pairs, encoding 62 amino acids. The partial gene had C-terminal conserved domains of substrate binding pocket (H-site) of GSTs superfamily. Homology comparison with GSTs gene amino acid sequences in other plants showed that it was 91% identical to 19E50 of wheat, 90% to pk0078 and 88% to gstf6. It was named as MwGSTs.

分类号： S54